Active Recombinant Human LAG3 Protein, MIgG2a Fc & Avi-tagged, Biotinylated

• Cat.No.: LAG3-385H
• Product Overview: Recombinant Human LAG3 Protein, MIgG2a Fc & Avi-tagged, Biotinylated is expressed from human 293 cells (HEK293). It contains AA Leu 23 - Leu 450 (Accession # P18627-1). Predicted N-terminus: Leu 23. This protein carries a mouse IgG2a Fc tag at the C-terminus, followed by an Avi tag. The protein has a calculated MW of 75.4 kDa. The protein migrates as 100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

Specification

• Source: HEK293
• Species: Human
• Tag: MIgG2a Fc & Avi
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Madarex LAG-3 Mab,Human IgG1 at 2 μg/mL (100 μL/well) can bind Recombinant Human LAG3 Protein, MIgG2a Fc & Avi-tagged, Biotinylated with a linear range of 0.4-13 ng/mL (QC tested).
  Flow Cytometry assay shows that Recombinant Human LAG3 Protein, MIgG2a Fc & Avi-tagged, Biotinylated can bind to daudi cell surface. The concentration of LAG-3 used is 0.3 μg/ml (Routinely tested).
• Molecular Mass: 75.4 kDa
• Protein length: Leu 23 - Leu 450
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Notes: Please avoid repeated freeze-thaw cycles.
• Stability: No activity loss is observed after storage at:
  4-8 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: LAG3 lymphocyte-activation gene 3 [ Homo sapiens ]
• Official Symbol: LAG3
• Synonyms: LAG3; lymphocyte-activation gene 3; lymphocyte activation gene 3 protein; CD223
• Gene ID: 3902
• mRNA Refseq: NM_002286
• Protein Refseq: NP_002277
• MIM: 153337
• UniProt ID: P18627