Active Recombinant Human TNFRSF17 Protein, MIgG2a Fc-tagged, low endotoxin

• Cat.No.: TNFRSF17-321H
• Product Overview: Recombinant Human TNFRSF17 Protein, MIgG2a Fc-tagged, low endotoxin is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 54 (Accession # Q02223-1). Predicted N-terminus: Met 1. This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 32.8 kDa. The protein migrates as 35-42 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Specification

• Source: HEK293
• Species: Human
• Tag: MIgG2a Fc
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human BAFF, Fc Tag at 2 μg/mL (100 μL/well) can bind Recombinant Human TNFRSF17 protein, MIgG2a Fc-tagged, low endotoxin with a linear range of 0.2-0.8 ng/mL (QC tested).
  Recombinant Human TNFRSF17 protein, MIgG2a Fc-tagged, low endotoxin at 2 μg/mL (100 μL/well) can bind Anti-Human BCMA Mab with a linear range of 0.2-3 ng/mL (Routinely tested).
  Immobilized Human APRIL, Fc Tag at 5 μg/mL (100 μL/well) can bind Recombinant Human TNFRSF17 protein, MIgG2a Fc-tagged, low endotoxin with a linear range of 0.2-2 ng/mL (Routinely tested).
• Molecular Mass: 32.8 kDa
• Protein length: Met 1 - Ala 54
• Endotoxin: Less than 0.1 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Notes: Please avoid repeated freeze-thaw cycles.
• Stability: No activity loss is observed after storage at:
  4-8 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: TNFRSF17 tumor necrosis factor receptor superfamily, member 17 [ Homo sapiens ]
• Official Symbol: TNFRSF17
• Synonyms: TNFRSF17; BCMA; BCM; CD269; B-cell maturation factor
• Gene ID: 608
• mRNA Refseq: NM_001192
• Protein Refseq: NP_001183
• MIM: 109545
• UniProt ID: Q02223