PLA2G2A

Case study 1: Mingquan Zhang, 2022

Oncogenic K-ras is often activated in pancreatic ductal adenocarcinoma (PDAC) due to frequent mutation (>90%), which drives multiple cellular processes, including alterations in lipid metabolism associated with a malignant phenotype. However, the role and mechanism of the altered lipid metabolism in K-ras-driven cancer remains poorly understood.

In this study, using human pancreatic epithelial cells harboring inducible K-rasG12D (HPNE/K-rasG12D) and pancreatic cancer cell lines, the expression of phospholipase A2 group IIA (PLA2G2A) was upregulated by oncogenic K-ras. The elevated expression of PLA2G2A was also observed in pancreatic cancer tissues. Abrogation of PLA2G2A by siRNA or by pharmacological inhibition using tanshinone I significantly increased lipid peroxidation.

Fig1. ANC-1, AsPC-1, and HPNE/K-rasG12D cells (stably induced with doxycycline to express oncogenic K-ras for 1 month) were transfected with 50 nM siRNA against PLA2G2A for 72 h, and the PLA2G2A mRNA level was quantified by qRT-PCR.

Fig2. Correlation between K-ras and FASN expression, and between PLA2G2A and FASN expression using GEPIA database.

Case study 2: Michael S Kuefner, 2017

Secretory phospholipase A2 group IIA (PLA2G2A) is a member of a family of secretory phospholipases that have been implicated in inflammation, atherogenesis, and antibacterial actions. Here, the researchers evaluated the role of PLA2G2A in the metabolic response to a high fat diet.

C57BL/6 (BL/6) mice do not express PLA2g2a due to a frameshift mutation. They fed BL/6 mice expressing the human PLA2G2A gene (IIA+ mice) a fat diet and assessed the physiologic response. After 10 weeks on the high fat diet, the BL/6 mice were obese, but the IIA+ mice did not gain weight or accumulate lipid. The IIA+ mice had increased expression of uncoupling protein 1 (UCP1), sirtuin 1 (SIRT1), and PPARγ coactivator 1α (PGC-1α) in brown adipose tissue (BAT), suggesting that PLA2G2A activates mitochondrial uncoupling in BAT.

Fig3. PLA2G2A protein expression from livers of chow diet or HFD mice.

Fig4. PLA2G2A protein expression from BAT and WAT of mice fed only chow diet.

Fig1. Schematic illustration of the mechanisms by which the upregulation of PLA2G2A by oncogenic K-ras promotes PDAC cell survival. (Mingquan Zhang, 2022)

Fig2. Model of sPLA2-IIA function in FLS AA metabolism. (Katherine J Bryant, 2011)

PLA2G2A involved in several pathways and played different roles in them. We selected most pathways PLA2G2A participated on our site, such as Glycerophospholipid metabolism, Ether lipid metabolism, Arachidonic acid metabolism, which may be useful for your reference. Also, other proteins which involved in the same pathway with PLA2G2A were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.

PLA2G2A has several biochemical functions, for example, calcium ion binding, calcium-dependent phospholipase A2 activity, phospholipase A2 activity. Some of the functions are cooperated with other proteins, some of the functions could acted by PLA2G2A itself. We selected most functions PLA2G2A had, and list some proteins which have the same functions with PLA2G2A. You can find most of the proteins on our site.

PLA2G2A has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with PLA2G2A here. Most of them are supplied by our site. Hope this information will be useful for your research of PLA2G2A.

ALOX12; CRMP1; CRELD2; CEP70