Recombinant Human PLA2G2A, His-tagged

• Cat.No.: PLA2G2A-1031H
• Product Overview: Total 142 AA. MW: 15.8 kDa (calculated). N-Terminal His-tag, 16 extra AA (highlighted).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: Phospholipase A2, membrane associated is an enzyme that in humans is encoded by the PLA2G2A gene.
• Form: Filtered (0.4 um) and lyophilized in 0.5 mg/ml in 0.05M Acetate buffer pH 4.
• Research topic: Secreted phospholipases A2
• Purity: >95%
• Reconstitution: Add 0.1M Acetate buffer pH4 to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10 ug/ml. In higher concentrations the solubility of this antigen is limited. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
• Applications: Western blotting
• Quality Control Test: BCA to determine quantity of the protein; SDS PAGE to determine purity of the protein.
• Sequences of amino acids: MRGSHHHHHH GMASHMNLVN FHRMIKLTTG KEAALSYGFY GCHCGVGGRG SPKDATDRCC VTHDCCYKRL EKRGCGTKFL SYKFSNSGSR ITCAKQDSCR SQLCECDKAA ATCFARNKTT YNKKYQYYSN KHCRGSTPRC
• Storage: Store lyophilized protein at -20°C. Lyophilized protein remains stable until the expiry date when stored at -20°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after one week at 4°C.

Gene Information

• Gene Name: PLA2G2A phospholipase A2, group IIA (platelets, synovial fluid) [ Homo sapiens ]
• Official Symbol: PLA2G2A
• Synonyms: PLA2G2A; phospholipase A2, group IIA (platelets, synovial fluid); MOM1; PLA2; PLA2B; PLA2L; PLA2S; PLAS1; sPLA2; OTTHUMP00000002786; Phospholipase A2, membrane associated; EC=3.1.1.4; Group IIA phospholipase A2; GIIC sPLA2; Non-pancreatic secretory phospholipase A2; NPS-PLA2; Phosphatidylcholine 2-acylhydrolase 2A; RASF-A; EC 3.1.1.4
• Gene ID: 5320
• mRNA Refseq: NM_000300
• Protein Refseq: NP_000291
• MIM: 172411
• Uniprot ID: P14555
• Chromosome Location: 1p35
• Function: calcium ion binding; calcium-dependent phospholipase A2 activity; hydrolase activity; phospholipase A2 activity; phospholipid binding