KRT18

  • Official Full Name

    keratin 18

  • Overview

    KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
  • Synonyms

    KRT18; keratin 18; K18; CYK18; keratin, type I cytoskeletal 18; CK-18; keratin-18; cytokeratin 18; cytokeratin-18; cell proliferation-inducing protein 46; cell proliferation-inducing gene 46 protein;

  • Recombinant Proteins
  • Cell & Tissue Lysates
  • Protein Pre-coupled Magnetic Beads
  • Native Proteins
  • Bovine
  • Human
  • Mouse
  • Pig
  • Rat
  • Zebrafish
  • bovine liver
  • E.coli
  • HEK293
  • In Vitro Cell Free System
  • Insect Cell
  • Mammalian Cell
  • Wheat Germ
  • GST
  • His
  • His (Fc)
  • Avi
  • His|T7
  • N/A
  • N
  • T7|His|TEV cleavage site
Species Cat.# Product name Source (Host) Tag Protein Length Price
Human KRT18-12H Recombinant Human KRT18 protein, His-tagged E.coli His
Human KRT18-266H Recombinant Human Cytokeratin-18 , His-tagged E.coli His
Human KRT18-174H Recombinant Human KRT18 Protein, His-tagged E.coli His Ser2~His430
Human KRT18-464H Recombinant Human Keratin 18 E.coli N/A
Human KRT18-13H Recombinant Human KRT18 protein, T7/His-tagged E.coli T7/His/TEV cleavage site 1 a.a. - 100 a.a.
Human KRT18-27702TH Recombinant Human KRT18, His-tagged E.coli His
Human KRT18-4877HCL Recombinant Human KRT18 293 Cell Lysate HEK293 N/A
Human KRT18-4876HCL Recombinant Human KRT18 293 Cell Lysate HEK293 N/A
Human KRT18-487H Recombinant Human KRT18 protein, GST-tagged E.coli GST 127-430 aa
Human KRT18-4867H Recombinant Human KRT18 Protein, GST-tagged Wheat Germ GST
Human KRT18-4348H Recombinant Human KRT18 protein, His-tagged Insect Cell His 2-430aa
Human KRT18-3146H Recombinant Human KRT18 protein, His-tagged E.coli His 11-430aa
Human KRT18-5030H Recombinant Human KRT18 protein, His-tagged HEK293 His 2-430aa
Human KRT18-4393H Recombinant Human KRT18 Protein (Asp238-Leu396), N-His tagged E.coli N-His Asp238-Leu396
Human KRT18-3147H Recombinant Human KRT18 protein, His-tagged E.coli His 102-396aa
Human KRT18-3148H Recombinant Human KRT18 protein E.coli N/A 2-430aa
Human KRT18-5928HF Recombinant Full Length Human KRT18 Protein, GST-tagged In Vitro Cell Free System GST 430 amino acids
Human KRT18-1075H Recombinant Human KRT18 Protein, His-tagged E.coli N-His Ser2-His430
Human KRT18-1585HFL Recombinant Full Length Human KRT18 Protein, N-His-tagged E.coli His
Mouse KRT18-1745M Recombinant Mouse Krt18 Protein, His-tagged E.coli His
Mouse KRT18-8821M Recombinant Mouse KRT18 Protein Mammalian Cell His
Mouse Krt18-5841M Recombinant Mouse Krt18 protein, His-tagged E.coli His Met1~His423 (Accession # P05784)
Mouse KRT18-4914M Recombinant Mouse KRT18 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Mouse KRT18-4914M-B Recombinant Mouse KRT18 Protein Pre-coupled Magnetic Beads HEK293
Rat KRT18-3315R Recombinant Rat KRT18 Protein Mammalian Cell His
Rat Krt18-5843R Recombinant Rat Krt18 protein, His-tagged E.coli His Glu72~Gly380
Rat KRT18-2971R-B Recombinant Rat KRT18 Protein Pre-coupled Magnetic Beads HEK293
Rat KRT18-2971R Recombinant Rat KRT18 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Bovine KRT18-173B Native bovine KRT18 bovine liver N/A
Zebrafish KRT18-9605Z Recombinant Zebrafish KRT18 Mammalian Cell His
Pig KRT18-5842P Recombinant Pig KRT18 protein, His & T7-tagged E.coli His/T7 Arg1~Thr159 (Accession # A8D349)
  • Background
  • Quality Guarantee
  • Case Study
  • Involved Pathway
  • Protein Function
  • Interacting Protein

What is KRT18 protein?

KRT18 (keratin 18) gene is a protein coding gene which situated on the long arm of chromosome 12 at locus 12q13. KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. The KRT18 protein is consisted of 430 amino acids and its molecular mass is approximately 48.1 kDa.

What is the function of KRT18 protein?

KRT18 is an intermediate filament protein belonging to the keratin family. It is expressed in a variety of tissues, especially in epithelial cells. It forms microfilaments together with other keratin molecules to maintain the structural integrity and stability of cells, and can also interact with proteins in cells to participate in apoptosis and cell cycle regulation.

KRT18 Related Signaling Pathway

The KRT18 protein exerts a wide range of influences on the structure and function of cells by participating in multiple signaling pathways. But the KRT18 protein itself is not directly involved in significant signaling pathways. The phosphorylation of KRT18 protein is related to the PI3K/Akt signaling pathway and MAPK/ERK signaling pathway, and is involved in the regulation of cell proliferation, survival and metabolism. The KRT18 protein has also been found to play a role in the NF-κB signaling pathway, which is primarily involved in processes such as inflammation and apoptosis.

KRT18 Related Diseases

KRT18 protein has been associated with a variety of diseases, especially epidermal tumors, such as epithelial tumors, non-small cell lung cancer (NSCLC), head and neck squamous cell carcinoma (HNSCC), esophageal cancer, and gastric cancer. In addition, KRT18 has been found to play a key role in liver cell damage and necrosis and is therefore associated with liver diseases (such as fatty liver, hepatitis, etc.). In addition, KRT18 protein may be involved in the regulation of cell damage and inflammatory response in Hemolytic Uremic Syndrome (HUS).

KRT18-7.jpg

Fig1. SHh and K8/18 immunohistochemistry are useful in detecting ballooned hepatocytes, regardless of background liver disease, and improving pathological diagnosis accuracy. (Hironori Kusano, 2022)

Bioapplications of KRT18

As a tumor marker, KRT18 may provide a new target for cancer therapy. KRT18 can be used for early diagnosis, disease monitoring and prognosis assessment of liver disease.

Case study 1: Ruizan Shi, 2020

Induction of epithelial-mesenchymal transition (EMT) and cancer stem cell (CSC) characteristics underlie the development of metastasis, chemoresistance, and tumor recurrence in breast cancer. Downregulation of cytokeratin 18 (CK18) is a critical molecular event of EMT; however, its importance in the induction of EMT and CSC features has not been defined to date. This study aimed to investigate the biological significance and underlying molecular mechanisms of CK18 in inducing EMT phenotype and stemness properties of breast cancer cells.

CK18 expression was extremely low in highly metastatic, resistant, and transforming growth factor (TGF)-β1/tumor necrosis factor (TNF)-α-treated breast cancer cells with mesenchymal phenotype and increased expression of CSC markers. Depletion of CK18 promoted partial EMT and the acquisition of stemness properties in breast cancer MCF-7 cells. Mechanistically, CK18 interference in MCF-7 cells activated the Wnt/β-catenin signaling. In conclusion, downregulation of CK18 promotes partial EMT and enhances breast cancer stemness by increasing EpCAM expression partly via the Wnt/β-catenin pathway.

KRT18-3.jpg

Fig1. The expression of EMT markers (E-cadherin, N-cadherin, and Vimentin), stemness markers (Oct4, Klf4, and Sox2), and CK18 in MCF-7, MDA-MB-231, and MCF-7/MX cells was detected using western blot assay.

KRT18-4.jpg
Fig2. Expression of EpCAM (green) in MCF-7 and MCF-7-shCK18 cells was analyzed by immunofluorescence staining and DAPI (blue).

Case study 2: Kenta Soeta, 2020

Cytokeratin (CK) 18 is an intermediate filament protein that plays a major functional role in the integrity and mechanical stability of cells. CK18 is also cleaved by some caspases during apoptosis. Three-dimensional (3D)-cultured cancer cells are useful for cancer research as an intermediate model between in vitro cancer cell line cultures and in vivo tumors.

In this study, the researchers produced rat monoclonal antibodies (mAbs) through immunization of the lysate from 3D-cultured DLD-1 cells to elucidate a characteristic feature of a tumor, and the results showed that mAb 2H7 recognized human CK18.

KRT18-5.jpg

Fig3. Comparison of recognition of endogenous CK18 by mAb 2H7 and mouse mAb through immunoblotting using several human cell lines.

KRT18-6.jpg
Fig4. Immunofluorescence staining of mAb 2H7 and mouse mAb using DLD-1 cells. DLD-1 cells were immunostained with mAb 2H7 or anti-CK18 mAb.

KRT18 involved in several pathways and played different roles in them. We selected most pathways KRT18 participated on our site, such as Pathogenic Escherichia coli infection, which may be useful for your reference. Also, other proteins which involved in the same pathway with KRT18 were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.

Pathway Name Pathway Related Protein
Pathogenic Escherichia coli infectionWASL;TUBB7P;ROCK2;WAS;ARHGEF2;TUBA8;TUBB6;KRT18;TUBA1B

KRT18 has several biochemical functions, for example, poly(A) RNA binding, protein binding, scaffold protein binding. Some of the functions are cooperated with other proteins, some of the functions could acted by KRT18 itself. We selected most functions KRT18 had, and list some proteins which have the same functions with KRT18. You can find most of the proteins on our site.

Function Related Protein
poly(A) RNA bindingPDAP1;UBE2I;EXOSC8;TRMT6;MARK2;HTATSF1;NOM1;SR140;ZNF207
protein bindingCIAO1;MAP3K2;BCS1L;RAB11A;TIRAP;SRSF5;SNCA;PSMD6;IL24
scaffold protein bindingADCY6;PDE4D;KRT15;NOS3;LRP4;PANX1;NLGN3;NLRP1;NLGN4Y
structural molecule activityTUBA1C;CLTB;INAB;ADD1;LCE3C;LMNB1;TUBB;KRT80;KRT9

KRT18 has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with KRT18 here. Most of them are supplied by our site. Hope this information will be useful for your research of KRT18.

KRT8; YWHAZ; DNAJB6; TRADD

  • Reviews
  • Q&As

Customer Reviews (3)

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Reviews
01/17/2022

    The sensitivity is very high, the experimental results are very good, and the unnecessary trouble is reduced.

    10/31/2021

      No doping impurities, the experimental effect is very good, will be recommended to other laboratory students.

      01/03/2021

        Easy to operate, cost-effective.

        Q&As (6)

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        What is the relationship between KRT18 protein and nail diseases? 10/23/2022

        KRT18 protein may be associated with nail diseases, such as brittle nails, white spots, and longitudinal cracks. These diseases can affect the hardness and structure of the nails, causing them to appear brittle, deformed, and painful.

        What is the relationship between KRT18 protein and hair color? 06/15/2022

        KRT18 protein is not directly related to hair color. Hair color is mainly determined by melanocytes, while the KRT18 protein is mainly involved in the process of keratinization.

        How can genetic testing be used to understand the genetic variation of KRT18 protein? 04/01/2022

        Genetic testing can be done by analyzing DNA sequences to understand genetic variation in the KRT18 protein. These variants may affect the expression level and function of the KRT18 protein, thereby increasing the risk of certain diseases.

        How can lifestyle changes affect the function of KRT18 protein? 01/21/2021

        Lifestyle changes, such as eating a healthy diet, exercising moderately, and avoiding UV damage, can affect the function and expression levels of KRT18 protein. These alterations can promote the normal function of keratinocytes, which can help maintain healthy skin.

        How can KRT18 protein-related diseases be prevented? 08/15/2020

        Prevention of KRT18 protein-related diseases is mainly through maintaining a healthy lifestyle, such as a balanced diet, moderate exercise, maintaining water intake, and avoiding UV damage. In addition, regular skin and oral exams can also help detect potential problems early.

        What is the role of KRT18 protein in melanoma? 03/13/2020

        In melanoma, the KRT18 protein may play a role in promoting tumor growth and metastasis. This may be related to the proliferation and migration of melanoma cells.

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