Recombinant Human KRT18 Protein, His-tagged

• Cat.No.: KRT18-1075H
• Product Overview: Recombinant Human KRT18 Protein (Ser2-His430) with His tag was expressed in E. coli.

Specification

• Description: KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.
• Source: E. coli
• Species: Human
• Tag: N-His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 51.6 kDa; Accurate Molecular Mass: 52 kDa
• Protein length: Ser2-His430
• Purity: > 80%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: KRT18 keratin 18 [ Homo sapiens (human) ]
• Official Symbol: KRT18
• Synonyms: KRT18; keratin 18; K18; CK-18; CYK18; keratin, type I cytoskeletal 18; cell proliferation-inducing gene 46 protein; cytokeratin 18; keratin 18, type I
• Gene ID: 3875
• mRNA Refseq: NM_000224
• Protein Refseq: NP_000215
• MIM: 148070
• UniProt ID: P05783