RFK
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Official Full Name
riboflavin kinase
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Overview
Riboflavin kinase (RFK; EC 2.7.1.26) is an essential enzyme that catalyzes the phosphorylation of riboflavin (vitamin;B2) to form flavin mononucleotide (FMN), an obligatory step in vitamin B2 utilization and flavin cofactor synthesis;(Karthikeyan et al., 2003 (PubMed 12623014)). -
Synonyms
RFK; riboflavin kinase; FLJ11149; RIFK; flavokinase; 0610038L10Rik; ATP:riboflavin 5-phosphotransferase; RP11-422N19.2;
- Recombinant Proteins
- Cell & Tissue Lysates
- Protein Pre-coupled Magnetic Beads
- Chicken
- Human
- Mouse
- E.coli
- HEK293
- HEK293T
- Human
- Mammalian Cell
- GST
- His
- His (Fc)
- Avi
- Myc
- DDK
- N/A
| Species | Cat.# | Product name | Source (Host) | Tag | Protein Length | Price |
|---|---|---|---|---|---|---|
| Human | RFK-107H | Recombinant Human Riboflavin Kinase | E.coli | N/A |
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| Human | RFK-8617H | Recombinant Human RFK protein, His-tagged | E.coli | His | Met1-His155 |
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| Human | RFK-2260H | Recombinant Human RFK, GST-tagged | E.coli | GST | 1-162aa | |
| Human | RFK-30014TH | Recombinant Human RFK, His-tagged | E.coli | His |
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| Human | RFK-897H | Recombinant Human Riboflavin Kinase, His-tagged | Human | His |
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| Human | RFK-2407HCL | Recombinant Human RFK 293 Cell Lysate | HEK293 | N/A |
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| Human | RFK-1608H | Recombinant Human RFK Protein, Myc/DDK-tagged, C13 and N15-labeled | HEK293T | Myc/DDK |
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| Mouse | RFK-14102M | Recombinant Mouse RFK Protein | Mammalian Cell | His |
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| Mouse | Rfk-5478M | Recombinant Mouse Rfk Protein, Myc/DDK-tagged | HEK293T | Myc/DDK |
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| Mouse | RFK-7541M | Recombinant Mouse RFK Protein, His (Fc)-Avi-tagged | HEK293 | His (Fc)-Avi |
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| Mouse | RFK-7541M-B | Recombinant Mouse RFK Protein Pre-coupled Magnetic Beads | HEK293 |
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| Chicken | RFK-3243C | Recombinant Chicken RFK | Mammalian Cell | His |
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- Background
- Quality Guarantee
- Case Study
- Involved Pathway
- Protein Function
- Interacting Protein
- RFK Related Articles
Fig1. A schematic model illustrating the functional coupling of TNFR1 to NADPH oxidase by RFK.
What is RFK protein?
RFK (Riboflavin Kinase) gene is a protein coding gene which situated on the long arm of chromosome 9 at locus 9q21. The gene encodes protein called Riboflavin kinase (RFK) consisted of 155 amino acids. This protein is approximately 17.6 kDa, also known as flavokinase, belongs to the family of transferases, specifically those transferring phosphorus-containing groups (phosphotransferases) with an alcohol group as acceptor.
What is the function of RFK protein?
RFK is an enzyme that catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin-mononucleotide (FMN), hence it is a rate-limiting enzyme in the synthesis of FAD. RFK is essential for TNF-induced reactive oxygen species (ROS) production. Through its interaction with both TNFRSF1A and CYBA, RFK physically and functionally couples TNFRSF1A to NADPH oxidase. TNF-activation of RFK may enhance the incorporation of FAD in NADPH oxidase, a critical step for the assembly and activation of NADPH oxidase.
RFK Related Diseases
Dysfunctional RFK results in riboflavin deficiency, which further affects the synthesis of two coenzymes, FMN and FAD, leading to neurodegenerative diseases like Alzheimer's Disease.
Bioapplications of RFK
RFK may play a role in the protection of the nervous system and has potential implications for the treatment or prevention of diseases associated with neuronal ischemia, such as ischemic brain injury. RFK is a key enzyme in riboflavin metabolism and is specifically expressed in microglia which has important implications for understanding the physiological and pathological processes of the nervous system.
High Purity
Fig1. SDS-PAGE (RFK-1608H) (PROTOCOL for western blot)
Case Study 1: Mengran Zhang, 2023
Fig1. RFK is mainly located in microglia and its expression is increased in the inflammation-based cognitive impairment model.
Case Study 2: Mengran Zhang, 2023
Fig2. Representative blots and quantification showing RFK expression levels in Control and LPS-treated BV2 cells n = 3 per group.
Fig1. KMT2B motivates MIRI-induced cellular injury and ferroptosis by inducing RFK transcription and mediating the TNF-α/NOX2 axis.
RFK involved in several pathways and played different roles in them. We selected most pathways RFK participated on our site, such as Riboflavin metabolism, Metabolic pathways, which may be useful for your reference. Also, other proteins which involved in the same pathway with RFK were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.
| Pathway Name | Pathway Related Protein |
|---|---|
| Riboflavin metabolism | P22;TYR;FLAD1;BLVRB;RFK |
| Metabolic pathways | UMPS;G6PCA.2;MOGAT3;ATP6V1AA;MBOAT1;SMPD5;CYP11B1;NME3;P4HA1 |
RFK has several biochemical functions, for example, ATP binding, metal ion binding, riboflavin kinase activity. Some of the functions are cooperated with other proteins, some of the functions could acted by RFK itself. We selected most functions RFK had, and list some proteins which have the same functions with RFK. You can find most of the proteins on our site.
| Function | Related Protein |
|---|---|
| ATP binding | CAMK1DA;ATP2B3B;DGKB;STK30;MERTK;EIF4A2;SHPRH;UBE2M;UBE2U |
| metal ion binding | GLIS2B;NT5M;FTR54;POLM;ZNF703;ZNF296;Car9;MGRN1B;ALKBH4 |
| riboflavin kinase activity |
RFK has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with RFK here. Most of them are supplied by our site. Hope this information will be useful for your research of RFK.
RAB1A; kipA; DNAJB9
- Q&As
- Reviews
Q&As (5)
Ask a questionYes, RFK protein modulation may hold potential for therapeutic intervention in diseases related to its deficiency or dysregulation.
RFK protein's involvement in cellular metabolism makes it relevant in fields such as oncology, neurology, and mitochondrial disorders.
Generally, riboflavin supplementation is considered safe; however, high doses might lead to minor side effects like diarrhea or urine discoloration.
Treatment primarily involves riboflavin supplementation, aiming to restore adequate levels of active FMN and address the underlying deficiency.
Understanding RFK protein variants or deficiencies helps in tailoring riboflavin supplementation and treatments for individuals with specific genetic profiles or conditions.
Customer Reviews (3)
Write a reviewI highly recommend the RFK protein for various experimental applications.
It has demonstrated outstanding performance in ELISA, providing reliable and precise results.
It has been invaluable in protein electron microscopy structure analysis, facilitating detailed investigations and yielding valuable insights.
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