Recombinant Mouse Rfk Protein, Myc/DDK-tagged

• Cat.No.: Rfk-5478M
• Product Overview: Purified recombinant protein of mouse full-length riboflavin kinase (Rfk), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin-mononucleotide (FMN), hence rate-limiting enzyme in the synthesis of FAD. Essential for TNF-induced reactive oxygen species (ROS) production. Through its interaction with both TNFRSF1A and CYBA, physically and functionally couples TNFRSF1A to NADPH oxidase. TNF-activation of RFK may enhance the incorporation of FAD in NADPH oxidase, a critical step for the assembly and activation of NADPH oxidase.
• Molecular Mass: 17.9 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Rfk riboflavin kinase [ Mus musculus (house mouse) ]
• Official Symbol: Rfk
• Synonyms: RFK; riboflavin kinase; flavokinase; ATP:riboflavin 5-phosphotransferase; KOI-4; AF031381; 0610038L10Rik
• Gene ID: 54391
• mRNA Refseq: NM_019437
• Protein Refseq: NP_062310
• UniProt ID: Q8CFV9