Recombinant Human Riboflavin Kinase

• Cat.No.: RFK-107H
• Product Overview: Recombinant riboflavin kinase was expressed inE.coliand purified by using conventional chromatography techniques. MW = 20.5 kDa (182 aa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-162 a.a.
• Description: Riboflavin kinase, also known as flavokinase, belongs to the family of transferases, specifically those transferring phosphorus-containing groups (phosphotransferases) with an alcohol group as acceptor. It is an enzyme that catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin-mononucleotide (FMN).
• Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MPRADCIMRH LPYFCRGQVV RGFGRGSKQL GIPTANFPEQ VVDNLPADIS TGIYYGWASV GSGDVHKMVV SIGWNPYYKN TKKSMETHIM HTFKEDFYGE ILNVAIVGYL RPEKNFDSLE SLISAIQGDI EEAKKRLELP EHLKIKEDNF FQVSKSKIMN GH.
• Form: Liquid. In 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol.
• Purity: > 90% by SDS – PAGE.
• Concentration: 1.0 mg/ml (determined by Bradford assay).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: RFK riboflavin kinase [ Homo sapiens ]
• Synonyms: RFK; riboflavin kinase; RIFK; FLJ11149; RP11-422N19.2; flavokinase; 0610038L10Rik; ATP:riboflavin 5"-phosphotransferase; EC 2.7.1.26; Flavokinase
• Gene ID: 55312
• mRNA Refseq: NM_018339
• Protein Refseq: NP_060809
• MIM: 613010
• UniProt ID: Q969G6
• Chromosome Location: 9q21.31
• Pathway: Metabolic pathways; Riboflavin metabolism; Metabolism of vitamins and cofact
• Function: ATP binding; magnesium ion binding; nucleotide binding; riboflavin kinase activity; riboflavin kinase activity; transferase activity; zinc ion binding