Recombinant Human S100A4, His-tagged

• Cat.No.: S100A4-3727H
• Product Overview: Total 110 AA. MW: 12.84 kDa (calculated). N-Terminal His-tag, 10 extra AA (highlighted).

Specification

• Cat. No.: S100A4-3727H
• Description: Protein S100-A4 is a protein that in humans is encoded by the S100A4 gene. The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified.
• Source: E. coli
• Species: Human
• Form: Filtered (0,4 μm) and lyophilized in 0.5 mg/mL in 20mM TRIS, 50mM NaCl, pH 7.5.
• Reconstitution: Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
• Applications: Western blotting
• Quality Control Test: BCA to determine quantity of the protein; SDS PAGE to determine purity of the protein
• Sequences of amino acids: MKHHHHHHAS ACPLEKALDV MVSTFHKYSG KEGDKFKLNK SELKELLTRE LPSFLGKRTD EAAFQKLMSN LDSNRDNEVD FQEYCVFLSC IAMMCNEFFE GFPDKQPRKK
• Storage: Store lyophilized protein at -80°C. Lyophilized protein remains stable until the expiry date when stored at -80°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term storage. Reconstituted protein can be stored at 4°C for a week.

Gene Information

• Gene Name: S100A4 S100 calcium binding protein A4 [ Homo sapiens ]
• Official Symbol: S100A4
• Synonyms: S100A4; S100 calcium binding protein A4; 42A; 18A2; CAPL; FSP1; MTS1; P9KA; PEL98; protein S100-A4; protein Mts1; OTTHUMP00000015467; OTTHUMP00000015468; fibroblast-specific protein-1; placental calcium-binding protein; malignant transformation suppression 1; leukemia multidrug resistance associated protein; S100 calcium-binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog); S100 calcium binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog); Protein S100-A4; Calvasculin; Metastasin; Placental calcium-binding protein; Protein Mts1; S100 calcium-binding protein A4
• Gene ID: 6275
• mRNA Refseq: NM_002961
• Protein Refseq: NP_002952
• MIM: 114210
• UniProt ID: P26447
• Chromosome Location: 1q12-q22
• Function: RAGE receptor binding; calcium ion binding; calcium-dependent protein binding; identical protein binding; protein binding

Reviews

10/07/2022

I have been using these for several years and they have been very stable and I swear by this product.

04/14/2022

The experimental process is simple and there is no special environmental requirement.

11/20/2021

It can remain stable under different temperature and pH conditions.

Q&As

What is the current progress in drug development for S100A4?

Drug development for S100A4 is currently in clinical trials, including small molecule inhibitors against S100A4 and inhibitors against the S100A4 signaling pathway.

Is the expression of S100A4 affected by environmental factors?

Yes, the expression of S100A4 is affected by environmental factors, such as nutritional status, inflammation level, etc.

What role does S100A4 play in tumor immunotherapy?

S100A4 plays an important role in tumor immunotherapy, it can act as a tumor-associated antigen, activate the immune response of tumor cells, promote apoptosis of tumor cells and inhibit the proliferation of tumor cells.

How to improve the nutritional status of S100A4 protein through diet?

There is no conclusive evidence that diet improves the nutritional status of S100A4 protein, but maintaining a balanced diet and moderate protein intake can help maintain good health.

How can S100A4 be used as a biomarker in clinical practice?

In clinical practice, the expression level of S100A4 can be detected to predict disease progression and prognosis, and to guide the choice of treatment regimen. For example, in the diagnosis and treatment of oncological diseases.

What is the relationship between S100A4 protein and muscle wasting?

Studies have shown that S100A4 protein can inhibit the occurrence of muscle wasting, but the specific mechanism is not clear.