Recombinant Human TYRO3 protein, DDK/His-tagged

• Cat.No.: TYRO3-195H
• Product Overview: Recombinant Human TYRO3(Ala41-Trp429) fused with DDK/His tag at C-terminal was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Flag&His
• Protein Length: 41-429 a.a.
• Description: The gene is part of a 3-member transmembrane receptor kinase receptor family with a processed pseudogene distal on chromosome 15. The encoded protein is activated by the products of the growth arrest-specific gene 6 and protein S genes and is involved in controlling cell survival and proliferation, spermatogenesis, immunoregulation and phagocytosis. The encoded protein has also been identified as a cell entry factor for Ebola and Marburg viruses.
• Form: 1 x PBS, pH7.4, 10% glycerol.
• Molecular Mass: 44 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >50 ug/mL as determined by microplate BCA method
• Publications:
  Harnessing MerTK agonism for targeted therapeutics (2020)

Gene Information

• Gene Name: TYRO3 TYRO3 protein tyrosine kinase [ Homo sapiens ]
• Official Symbol: TYRO3
• Synonyms: TYRO3; TYRO3 protein tyrosine kinase; RSE; tyrosine-protein kinase receptor TYRO3; Brt; Dtk; Sky; Tif; tyrosine-protein kinase DTK; tyrosine-protein kinase RSE; tyrosine-protein kinase SKY; tyrosine-protein kinase byk; BYK; FLJ16467
• Gene ID: 7301
• mRNA Refseq: NM_006293
• Protein Refseq: NP_006284
• MIM: 600341
• UniProt ID: Q06418
• Chromosome Location: 15q15.1-q21.1
• Function: ATP binding; nucleotide binding; phosphatidylinositol 3-kinase binding; protein binding; protein heterodimerization activity; protein tyrosine kinase activity; receptor activity; receptor signaling protein tyrosine kinase activity; transmembrane receptor protein tyrosine kinase activity

Citation

Harnessing MerTK agonism for targeted therapeutics

Journal: mAbs    PubMed ID: 31852344    Data: 2022/12/14

Authors: Vivekananda Kedage, Diego Ellerman, Minhong Yan

Article Snippet:ELISA was carried out to determine the specificity of MerTK bispecific antibodies for other members of TAM family.ELISA was carried out to determine the specificity of MerTK bispecific antibodies for other members of TAM family.. Briefly, 1ug/ml of human MerTK (Genentech), human AXL (Creative BioMart, Catalog AXL-769H), human TYRO3 (Creative BioMart, Catalog TYRO3-195H), mouse MerTK (R&D systems, Catalog 591-MR-100), mouse AXL (Creative BioMart, Catalog AXL-626M), mouse TYRO3 (R&D systems, Catalog 759-DT-100) were coated onto ELISA plates.. The plates were blocked with SuperBlock (TBS) blocking buffer (Thermo Fisher, Catalog 37535). hIgG1 and mIgG2a versions of bispecific antibodies were added followed by horseradish peroxidase (HRP)-conjugated anti-human IgG (Jackson laboratories, Catalog 709-035-149) and HRP-conjugated anti-mouse IgG (Jackson laboratories, Catalog 715-035-151) at 1:20,000 dilution.The plates were blocked with SuperBlock (TBS) blocking buffer (Thermo Fisher, Catalog 37535). hIgG1 and mIgG2a versions of bispecific antibodies were added followed by horseradish peroxidase (HRP)-conjugated anti-human IgG (Jackson laboratories, Catalog 709-035-149) and HRP-conjugated anti-mouse IgG (Jackson laboratories, Catalog 715-035-151) at 1:20,000 dilution.