6-Phosphofructokinase Activity Assay Kit
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Cat.No. : | Kit-0678 |
Product Overview : | 6-Phosphofructokinase Activity Assay kit, PFK (phosphofructokinase) converts fructose-6-phosphate and ATP to fructose-diphosphate and ADP. The ADP in the presence of substrate and enzyme mix is converted to AMP and NADH, which reduces a colorless probe to a colored product with strong absorbance at 450 nm. PFK activity assay is simple, robust, and rapid and can detect phosphofructokinase activity less than 1 mU. |
Applications : | Functional Studies |
Storage : | Store the kit at-20°C and protect from light. Please read the entire protocol before performing the assay. Avoid repeated freeze/thaw cycles. Warm all Buffers to room temperature before use. Briefly centrifuge all small vials prior to opening. |
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