Actin Binding Protein Assay Kit: rabbit skeletal muscle actin
Cat.No. : | Kit-0046 |
Product Overview : | The Actin Binding Protein Spin-Down Biochem Kit is an extremely quick and economical way to obtain an answer concerning binding affinity for monomer (G-) or polymer (F-) actin. If you are new to the field you may not know that actin requires ATP and a divalent cation for stability. Without this knowledge it is easy to obtain incorrect data, which can lead to inappropriate experimental interpretation. This kit is designed to guide you through the process of studying actin binding proteins The Actin Binding Protein Spin-Down Biochem Kit provides G-or F-actin plus positive ( -actinin) and negative (Bovine Serum Albumin, BSA) binding control proteins. Actin binding occurs when there is an affinity for any site of actin. F-actin binding can be measured by using a spin down assay where centrifugation is used to separate F-actin from G-actin by differential sedimentation. F-actin binding proteins will co-sediment with actin filaments and form a pellet at the bottom of the centrifugation tube. F-actin severing proteins, G-actin binding proteins or non-actin binding proteins will stay in the supernatant. Actin severing proteins will result in more G-actin remaining in the supernatant compared to the negative control sample. This activity should be further tested by measuring F-actin length distributions before and after adding the test protein. G-actin binding proteins can be measured by adding the test protein to G-actin and inducing polymerization. If the test protein sequesters G-actin, more actin will remain in the supernatant compared with the control. Actin can exist in two forms: Globular subunit (G-actin) and Filamentous polymer (F-actin). Both forms of actin interact with a plethora of proteins in the cell. To date there are over 50 distinct classes of Actin-Binding Proteins (ABPs) and the inventory is still far from complete. Actin Binding Proteins allow the actin cytoskeleton to respond rapidly to cellular and extracellular signals and are integral to cytoskeletal involvement in many cellular processes. These include cell shape and motility, muscle contraction, intracellular trafficking, cell pathogenesis and signal transduction. A comprehensive review of the huge body of literature concerning the structure and functions of ABPs is beyond the scope of this general introduction and we direct the reader to several excellent review articles and references therein. In this introduction to ABPs we will briefly outline the major recognized classes of ABPs and the experimental procedures that are currently used to study ABP activity. |
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Size : | 30-100 assays |
Applications : | 1. To determine whether a protein binds to filaments or monomers of actin.2. To determine whether a protein bundles F-actin.3. To test various conditions (e.g. pH optima) or requirements (e.g. divalent cation requirement) for binding to actin. |
Products Types
◆ Native Protein | ||
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Actin-3084R | Active Native Rabbit Actin Protein | +Inquiry |
◆ Assay kits | ||
Kit-0045 | Actin Binding Protein Assay Kit: human platelet actin | +Inquiry |
Kit-0047 | Actin Polymerization Kit: rabbit skeletal muscle actin | +Inquiry |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (20)
Ask a questionYes, actin structures can undergo rearrangements and changes in response to external cues such as growth factors, mechanical forces, or chemical signals.
Actin-based structures, like lamellipodia and filopodia, play a crucial role in cell migration by generating forces and promoting protrusion and adhesion.
Actin forms structures such as stress fibers, filopodia, lamellipodia, and contractile rings, among others.
Actin structures interact with and are critical for the function and regulation of factors involved in mRNA and protein production, degradation, and regulation.
Actin filaments, in coordination with adhesive proteins, contribute to cell adhesion and migration processes by mediating interactions with the extracellular matrix and promoting cellular movement.
Yes, actin dynamics can be regulated by various external factors and signaling pathways, leading to changes in cell behavior and protein regulation.
Yes, actin-related diseases include cytoskeletal disorders such as muscular dystrophies and neurodevelopmental disorders like Wiskott-Aldrich syndrome.
Actin's involvement in protein regulation includes influencing mRNA transcription, protein production, degradation, and overall protein homeostasis.
Actin filaments provide structural support to the cell, determining its shape and maintaining its integrity.
Actin is a key regulator of protein homeostasis across eukaryotes, helping maintain proper protein levels and function in the cell.
Cellular stress can alter actin's roles and functions, impacting processes from mRNA transcription to protein degradation.
Actin can act as a scaffold for signaling molecules and be directly involved in signaling cascades, influencing cell behavior and gene expression.
Yes, mutations in actin genes have been linked to various diseases, including cardiac and skeletal muscle disorders, intellectual disabilities, and hearing loss.
Actin and its regulatory proteins are potential targets for therapeutic interventions aimed at modulating cytoskeletal dynamics and addressing related diseases.
Cellular stress can lead to alterations in actin cytoskeleton organization and dynamics, affecting its roles in protein regulation and overall cellular function.
Actin-based structures, such as stress fibers and actin rings, are involved in protein degradation processes such as autophagy and proteasomal degradation.
Yes, actin is sometimes overlooked despite being a crucial regulator of protein homeostasis across eukaryotes.
Actin structures are involved in mRNA transport, localization, and translation, contributing to the regulation of gene expression in the cell.
Actin serves as a major constituent of the cytoskeleton and is involved in forming various cellular structures.
Actin is involved in various stages of cell division, including cytokinesis, where it forms the contractile ring that helps separate the dividing cells.
Customer Reviews (5)
Write a reviewWe received comprehensive documentation and technical datasheets with the protein product, facilitating its proper usage.
The protein exhibits robust enzymatic activity, leading to efficient and precise biochemical reactions.
The protein product is highly soluble and easily resuspended, allowing for convenient experimental use.
The batch-to-batch variability of the protein product is minimal, providing confidence in its reliability for long-term experiments.
The packaging included clear labeling and instructions, simplifying the handling and storage of the protein product.
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