Active Recombinant COVID-19 Spike NTD protein(BA.2/Omicron), His-Avi-tagged, Biotinylated

• Cat.No.: Spike-745V
• Product Overview: Biotinylated Recombinant COVID-19 Spike NTD protein(BA.2/Omicron)(Ser 13 - Leu 303)(Accession # QHD43416.1(T19I, LPP24-26del, A27S, G142D, V213G)), fused with His and Avi tag at the C-terminus, was expressed in HEK293.

Specification

• Source: HEK293
• Species: COVID-19
• Tag: His-Avi
• Form: Lyophilized from 0.22 μm filtered solution in PBS. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Biotinylated SARS-CoV-2 Spike NTD, His,Avitag (BA.2/Omicron) at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Anti-SARS-CoV-2 Spike NTD Antibody, Chimeric mAb, Human IgG1 with a linear range of 0.1-2 ng/mL.
• Molecular Mass: The protein has a calculated MW of 36.3 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: Ser 13 - Leu 303
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
  >90% as determined by SEC-MALS.
• Storage: This product is stable after storage at:
  -20°C to -70°C for 12 months in lyophilized state;
  -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: S surface glycoprotein [ Severe acute respiratory syndrome coronavirus 2 ]
• Official Symbol: Spike
• Synonyms: Spike; S1 protein; Spike glycoprotein Subunit1; S glycoprotein Subunit1; Spike protein S1; COVID-19 Omicron
• Gene ID: 43740568
• Protein Refseq: YP_009724390.1
• UniProt ID: P0DTC2