Recombinant COVID-19 S protein RBD, His-tagged (MALS verified)
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|Product Overview :||Recombinant COVID-19 S protein RBD (MALS verified) (Arg319-Lys537) was fused to His-tag at the C-terminus and expressed in human 293 cells (HEK293).|
|Description :||It's been reported that COVID-19 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.|
|Predicted N Terminal :||Arg319|
|Form :||Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.|
|Molecular Mass :||The protein has a calculated MW of 26.5 kDa. The protein migrates as 32-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.|
|Protein length :||Arg319-Lys537|
|Endotoxin :||Less than 1.0 EU per μg by the LAL method.|
|Purity :||>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
|Storage :||For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
|Reconstitution :||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.|
|SDS-PAGE of Spike-212V :||
COVID-19 S protein RBD, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
|SEC-MALS of Spike-212V :||
The purity of COVID-19 S protein RBD, His Tag was more than 90% and around 30-40 kDa verified by SEC-MALS.
|Bioactivity-ELISA of Spike-212V :||
Immobilized Human ACE2, Fc Tag at 2 μg/mL (100 μL/well) can bind COVID-19 S protein RBD, His Tag with a linear range of 8-125 ng/mL (QC tested).
Immobilized COVID-19 S protein RBD, His Tag at 1 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag with a linear range of 0.2-3 ng/mL (Routinely tested).
Immobilized COVID-19 S protein RBD, His Tag at 1 μg/mL (100 μL/well) can bind Anti-COVID-19 Neutralizing Antibody, Human IgG1 with a linear range of 0.1-3 ng/mL (Routinely tested).
|Bioactivity-SPR of Spike-212V :||
Human ACE2, Fc Tag captured on CM5 chip via anti-human IgG Fc antibodies surface can bind COVID-19 S protein RBD, His Tag with an affinity constant of 17 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
|Bioactivity-BLI of Spike-212V :||
Loaded Human ACE2, Fc Tag on Protein A Biosensor, can bind COVID-19 S protein RBD, His Tag with an affinity constant of 34.5 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
|Gene Name :||S|
|Official Symbol :||S|
|Synonyms :||spike glycoprotein|
|Gene ID :||43740568|
|Protein Refseq :||YP_009724390.1|
|UniProt ID :||P0DTC2|
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