Recombinant COVID-19 S protein RBD, His-tagged (MALS verified)

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Recombinant COVID-19 S protein RBD, His-tagged (MALS verified)

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Cat.No. : Spike-212V
Product Overview : Recombinant COVID-19 S protein RBD (MALS verified) (Arg319-Lys537) was fused to His-tag at the C-terminus and expressed in human 293 cells (HEK293).
Description : It's been reported that COVID-19 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Source : HEK293
Species : COVID-19
Tag : His
Predicted N Terminal : Arg319
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 26.5 kDa. The protein migrates as 32-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length : Arg319-Lys537
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE of Spike-212V :

COVID-19 S protein RBD, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS of Spike-212V :

The purity of COVID-19 S protein RBD, His Tag was more than 90% and around 30-40 kDa verified by SEC-MALS.

Bioactivity-ELISA of Spike-212V :

Immobilized Human ACE2, Fc Tag at 2 μg/mL (100 μL/well) can bind COVID-19 S protein RBD, His Tag with a linear range of 8-125 ng/mL (QC tested).

Immobilized COVID-19 S protein RBD, His Tag at 1 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag with a linear range of 0.2-3 ng/mL (Routinely tested).

Immobilized COVID-19 S protein RBD, His Tag at 1 μg/mL (100 μL/well) can bind Anti-COVID-19 Neutralizing Antibody, Human IgG1 with a linear range of 0.1-3 ng/mL (Routinely tested).

Bioactivity-SPR of Spike-212V :

Human ACE2, Fc Tag captured on CM5 chip via anti-human IgG Fc antibodies surface can bind COVID-19 S protein RBD, His Tag with an affinity constant of 17 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

Bioactivity-BLI of Spike-212V :

Loaded Human ACE2, Fc Tag on Protein A Biosensor, can bind COVID-19 S protein RBD, His Tag with an affinity constant of 34.5 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Gene Name : S
Official Symbol : S
Synonyms : spike glycoprotein
Gene ID : 43740568
Protein Refseq : YP_009724390.1
UniProt ID : P0DTC2

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