Active Recombinant Human EPCAM protein, Fc-tagged

• Cat.No.: EPCAM-475H
• Product Overview: Recombinant Human EPCAM(Gln24 - Lys265) fused with Fc tag at C-terminal was expressed in NS0.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: Fc
• Predicted N Terminal: Gln24
• Form: Lyophilized from a 0.2 μm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of the L Cells mouse fibroblast cell line.When 5 x 10^4 cells/well are added to recombinant human EpCAM/Fc Chimera and human Fibronectin (0.5 μg/mL) coated plates, cell adhesion is enhanced in a dose dependent manner after 45 minutes at 37 centigrade. The ED50 for this effect is typically 0.7-2.8 μg/mL. Measured by its binding ability in a functional ELISA. The activity of this protein is measured in a homophilic binding assay.
• Molecular Mass: Predicted Molecular Mass: 54 (monomer) kDa;SDS-PAGE: 60-70 kDa, reducing conditions
• Protein length: Gln24 - Lys265
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Gene Information

• Gene Name: EPCAM epithelial cell adhesion molecule [ Homo sapiens ]
• Official Symbol: EPCAM
• Synonyms: EPCAM; epithelial cell adhesion molecule; antigen identified by monoclonal AUA1 , M4S1, MIC18, TACSTD1, tumor associated calcium signal transducer 1; 17 1A; 323/A3; CD326; CO 17A; EGP 2; EGP34; EGP40; Ep CAM; ESA; GA733 2; HEA125; KS1/4; KSA; Ly74; MH99; MK 1; MOC31; TACST 1; TROP1; epithelial glycoprotein 314; human epithelial glycoprotein-2; cell surface glycoprotein Trop-1; adenocarcinoma-associated antigen; tumor-associated calcium signal transducer 1; major gastrointestinal tumor-associated protein GA733-2; membrane component, chromosome 4, surface marker (35kD glycoprotein); M4S1; MK-1; DIAR5; EGP-2; MIC18; EGP314; HNPCC8; TACSTD1; GA733-2
• Gene ID: 4072
• mRNA Refseq: NM_002354
• Protein Refseq: NP_002345
• MIM: 185535
• UniProt ID: P16422
• Chromosome Location: 2p21
• Function: protein binding