Active Recombinant Human IDE Protein (42-1019aa), C-His tagged
Cat.No. : | IDE-07H |
Product Overview : | Recombinant human Insulysin/IDE (42-1019aa), fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques. |
- Specification
- Gene Information
- Related Products
Description : | This gene encodes a zinc metallopeptidase that degrades intracellular insulin, and thereby terminates insulins activity, as well as participating in intercellular peptide signalling by degrading diverse peptides such as glucagon, amylin, bradykinin, and kallidin. The preferential affinity of this enzyme for insulin results in insulin-mediated inhibition of the degradation of other peptides such as beta-amyloid. Deficiencies in this protein's function are associated with Alzheimer's disease and type 2 diabetes mellitus but mutations in this gene have not been shown to be causitive for these diseases. This protein localizes primarily to the cytoplasm but in some cell types localizes to the extracellular space, cell membrane, peroxisome, and mitochondrion. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described but have not been experimentally verified. |
Source : | Baculovirus |
Species : | Human |
Tag : | C-His |
Form : | Liquid |
Bio-activity : | > 3,000 pmol/min/μg in which one unit will convert 1.0 pmole of Mca-RPPGFSAFK(Dnp)-OH to MCA-Pro-Leu-OH per minute at pH 7.5 at 25 centigrade. |
Molecular Mass : | 114 kDa (984aa) |
Protein Length : | 42-1019aa |
AA Sequence : | MNNPAIKRIGNHITKSPEDKREYRG LELANGIKVLLISDPTTDKSSAALD VHIGSLSDPPNIAGLSHFCEHMLFL GTKKYPKENEYSQFLSEHAGSSNAF TSGEHTNYYFDVSHEHLEGALDRFA QFFLCPLFDESCKDREVNAVDSEHE KNVMNDAWRLFQLEKATGNPKHPFS KFGTGNKYTLETRPNQEGIDVRQEL LKFHSAYYSSNLMAVCVLGRESLDD LTNLVVKLFSEVENKNVPLPEFPEH PFQEEHLKQLYKIVPIKDIRNLYVT FPIPDLQKYYKSNPGHYLGHLIGHE GPGSLLSELKSKGWVNTLVGGQKEG ARGFMFFIINVDLTEEGLLHVEDII LHMFQYIQKLRAEGPQEWVFQECKD LNAVAFRFKDKERPRGYTSKIAGIL HYYPLEEVLTAEYLLEEFRPDLIEM VLDKLRPENVRVAIVSKSFEGKTDR TEEWYGTQYKQEAIPDEVIKKWQNA DLNGKFKLPTKNEFIPTNFEILPLE KEATPYPALIKDTAMSKLWFKQDDK FFLPKACLNFEFFSPFAYVDPLHCN MAYLYLELLKDSLNEYAYAAELAGL SYDLQNTIYGMYLSVKGYNDKQPIL LKKIIEKMATFEIDEKRFEIIKEAY MRSLNNFRAEQPHQHAMYYLRLLMT EVAWTKDELKEALDDVTLPRLKAFI PQLLSRLHIEALLHGNITKQAALGI MQMVEDTLIEHAHTKPLLPSQLVRY REVQLPDRGWFVYQQRNEVHNNCGI EIYYQTDMQSTSENMFLELFCQIIS EPCFNTLRTKEQLGYIVFSGPRRAN GIQGLRFIIQSEKPPHYLESRVEAF LITMEKSIEDMTEEAFQKHIQALAI RRLDKPKKLSAECAKYWGEIISQQY NFDRDNTEVAYLKTLTKEDIIKFYK EMLAVDAPRRHKVSVHVLAREMDSC PVVGEFPCQNDINLSQAPALPQPEV IQNMTEFKRGLPLFPLVKPHINFMA AKL |
Endotoxin : | < 1 EU/μg of protein (determined by LAL method) |
Purity : | > 90% by SDS-PAGE |
Applications : | SDS-PAGE, Enzyme Activity |
Storage : | Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles. |
Concentration : | 0.5 mg/mL (determined by Absorbance at 280nm) |
Storage Buffer : | 20mM Tris-HCl buffer (pH 7.5) containing 100mM NaCl, 0.05% Brij35, 10% glycerol |
Gene Name : | IDE insulin degrading enzyme [ Homo sapiens (human) ] |
Official Symbol : | IDE |
Synonyms : | IDE; insulin degrading enzyme; INSULYSIN; insulin-degrading enzyme; Abeta-degrading protease; insulin protease; insulinase; EC 3.4.24.56 |
Gene ID : | 3416 |
mRNA Refseq : | NM_004969 |
Protein Refseq : | NP_004960 |
MIM : | 146680 |
UniProt ID : | P14735 |
Products Types
◆ Recombinant Protein | ||
IDE-1133H | Recombinant Human IDE Protein, His (Fc)-Avi-tagged | +Inquiry |
Ide-1644M | Recombinant Mouse Ide Protein, His-tagged | +Inquiry |
IDE-2639R | Recombinant Rat IDE Protein, His (Fc)-Avi-tagged | +Inquiry |
IDE-2011R | Recombinant Rhesus Macaque IDE Protein, His (Fc)-Avi-tagged | +Inquiry |
Ide-1211M | Recombinant Mouse Ide Protein, MYC/DDK-tagged | +Inquiry |
◆ Lysates | ||
IDE-5308HCL | Recombinant Human IDE 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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