Active Recombinant Human IL17A & IL17F Heterodimer Protein, His & Strep II-tagged

• Cat.No.: IL17A&IL17F-338H
• Product Overview: Recombinant Human IL17A & IL17F Heterodimer Protein, His & Strep II-tagged is expressed from human 293 cells (HEK293). It contains AA Gly 24 - Ala 155 (IL-17A) & Arg 31 - Gln 163 (IL-17F) (Accession # Q16552-1 (IL-17A) & Q96PD4-1 (IL-17F)). Two sequential affinity purification steps were used to ensure exact 1:1 molar ratio of IL17A ＆IL17F heterodimer. Predicted N-terminus: His (IL-17A) & Ser (IL-17F). Human IL-17A & IL-17F Heterodimer Protein, produced by co-expression of IL-17A and IL-17F, has a calculated MW of 17.0 kDa (IL-17A) and 18.4 kDa (IL-17F). Subunit IL-17A is fused with a polyhistidine tag at the N-terminus and subunit IL-17F is fused with a Twin Strep tag at the N-terminus. The predicted N-terminus is His (IL-17A) & Ser (IL-17F). The reducing (R) protein migrates as 19 kDa (IL-17A) and 21 kDa (IL-17F) respectively due to glycosylation.

Specification

• Source: HEK293
• Species: Human
• Tag: His & Strep II
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Recombinant Human IL17A & IL17F Heterodimer Protein, His & Strep II-tagged at 2 μg/mL (100 μL/well) can bind Human IL-17 RA, Fc Tag with a linear range of 39-625 ng/mL (QC tested).
  Immobilized Anti-IL17A Mab Human IgG1 at 2 μg/mL (100 μL/well) can bind Recombinant Human IL17A & IL17F Heterodimer Protein, His & Strep II-tagged with a linear range of 0.2-3 ng/mL (Routinely tested).
• Molecular Mass: 17.0 kDa (IL-17A) & 18.4 kDa (IL-17F)
• Protein length: Gly 24 - Ala 155 (IL-17A) & Arg 31 - Gln 163 (IL-17F)
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by reduced SDS-PAGE.
• Notes: Please avoid repeated freeze-thaw cycles.
• Stability: No activity loss is observed after storage at:
  4-8 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: IL17A interleukin 17A [ Homo sapiens ] & IL17F interleukin 17F [ Homo sapiens ]
• Official Symbol: IL17A & IL17F
• Synonyms: IL17A; interleukin 17A; IL 17; IL 17A; CTLA-8; IL17; CTLA8; IL-17; IL-17A; & IL17F; interleukin 17F; IL 17F; ML 1; ML1; IL-24; cytokine ML-1; ML-1; CANDF6; IL-17F
• Gene ID: 3605 & 112744
• mRNA Refseq: NM_002190 & NM_052872
• Protein Refseq: NP_002181 & NP_443104
• MIM: 603149 & 606496
• UniProt ID: Q16552 & Q96PD4