Active Recombinant Human IL6R protein, His/Avi-tagged, Biotinylated
Cat.No. : | IL6R-051H |
Product Overview : | Biotinylated Recombinant Human IL6R(Leu20-Pro365) protein, fused to His/Avi tag at the C-terminus, was expressed in CHO cells . |
- Specification
- Gene Information
- Related Products
Description : | Interleukin-6 receptor subunit alpha (IL-6Ra), also known as membrane glycoprotein 80 (gp80), IL-6R 1 or CD126, is a single-pass type I membrane protein of the interleukin receptor family. Human IL-6Ra is an 80 kDa receptor component that binds IL-6 with low affinity and forms one-half of the IL-6R receptor complex. IL-6Ra associates with a non-ligand binding 130 kDa glycoprotein signal-transducing component (CD130; gp130) for high-affinity binding to IL-6 (1-4). When bound to IL-6, the IL-6R complex forms a trimer, which homodimerizes to form a hexameric cellular signaling complex (1, 4). Human IL-6Ra consists of an extracellular domain (ECD) with an N-terminal Ig-like C2-type domain and a cytokine-binding domain containing two fibronectin type-III domains with a WSXWS motif, a helical transmembrane segment and a cytoplasmic domain. Within the mature ECD, human IL-6Ra shares 51% and 52% amino acid sequence identity with mouse and rat IL-6Ra, respectively. Soluble forms of IL-6Ra can be generated via ADAM10 and ADAM17 cleavage of membrane bound IL-6Ra, alternative mRNA splicing and microvesicle release (3, 4). Unlike gp130 that is expressed ubiquitously, the cellular distribution of IL-6 R alpha is predominantly limited to hepatocytes and leukocyte subpopulations such as monocytes, neutrophils, T and B cells. Soluble IL-6R alpha has been found in various body fluids (5). It has been documented that elevated soluble IL-6 R is associated with numerous diseases including arthritic lesions, multiple myeloma and Crohn's disease (6, 7). Our Avi-tag Biotinylated IL-6Ra features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity. |
Source : | CHO cells |
Species : | Human |
Tag : | His/Avi |
Predicted N Terminal : | Leu20 |
Form : | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
Bio-activity : | Measured by its binding ability in a functional ELISA. When Human IL6 is immobilized at 0.25 µg/mL (100 µL/ well), Biotinylated Recombinant Human IL6R His-tag Avi‑tag binds with an ED50 of 0.1-1 μg/mL. |
Molecular Mass : | 65-80 kDa, under reducing conditions |
Protein length : | Leu20-Pro365 |
Endotoxin : | <0.10 EU per 1 μg of the protein by the LAL method. |
Purity : | >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
Applications : | Bioactivity |
Storage : | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 °C as supplied.1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution. |
Reconstitution : | Reconstitute at 1 mg/mL in PBS. |
Gene Name : | IL6R interleukin 6 receptor [ Homo sapiens ] |
Official Symbol : | IL6R |
Synonyms : | IL6R; interleukin 6 receptor; interleukin-6 receptor subunit alpha; CD126; IL-6R 1; CD126 antigen; membrane glycoprotein 80; IL-6 receptor subunit alpha; gp80; IL6RA; IL-6RA; IL-6R-1; MGC104991; |
Gene ID : | 3570 |
mRNA Refseq : | NM_000565 |
Protein Refseq : | NP_000556 |
MIM : | 147880 |
UniProt ID : | P08887 |
Products Types
◆ Recombinant Protein | ||
IL6R-1046H | Recombinant Human IL6R protein(Met1-Pro365), hFc-tagged | +Inquiry |
IL6R-3154H | Recombinant Human IL6R Protein, His (Fc)-Avi-tagged | +Inquiry |
IL6R-457R | Active Recombinant Rhesus IL6R Protein, His-tagged | +Inquiry |
IL6R-070H | Recombinant Human IL6R Protein, C-His-tagged | +Inquiry |
IL6R-517H | Recombinant Human IL6R Protein, C13 and N15 Labeled | +Inquiry |
◆ Lysates | ||
IL6R-2903HCL | Recombinant Human IL6R cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
- Q&As
- Reviews
Q&As (6)
Ask a questionThe dose and route of administration need to be determined according to the specific treatment situation. Common routes of administration include subcutaneous injection and intravenous injection.
It can be used to treat certain autoimmune diseases, such as rheumatoid arthritis and psoriasis.
The IL6R is primarily used to treat inflammatory diseases that have already occurred, rather than prevent them.
IL6R reduces inflammation by binding to interleukin-6 (IL-6), blocking the IL-6 signaling pathway.
Although IL6R can regulate the immune system, it is not a direct anti-tumor drug.
The efficacy of IL6R is usually evaluated based on improvement of clinical symptoms, changes in disease activity, and changes in biochemical markers.
Customer Reviews (3)
Write a reviewGood compatibility and stability with the surfactant.
Expression level and stability of clones were good.
Remarkable antibacterial activity and bactericidal effect on bacteria.
Ask a Question for All IL6R Products
Required fields are marked with *
My Review for All IL6R Products
Required fields are marked with *
Inquiry Basket