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Active Recombinant Human IL6R protein, His/Avi-tagged, Biotinylated

Cat.No. : IL6R-051H
Product Overview : Biotinylated Recombinant Human IL6R(Leu20-Pro365) protein, fused to His/Avi tag at the C-terminus, was expressed in CHO cells .
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Description : Interleukin-6 receptor subunit alpha (IL-6Ra), also known as membrane glycoprotein 80 (gp80), IL-6R 1 or CD126, is a single-pass type I membrane protein of the interleukin receptor family. Human IL-6Ra is an 80 kDa receptor component that binds IL-6 with low affinity and forms one-half of the IL-6R receptor complex. IL-6Ra associates with a non-ligand binding 130 kDa glycoprotein signal-transducing component (CD130; gp130) for high-affinity binding to IL-6 (1-4). When bound to IL-6, the IL-6R complex forms a trimer, which homodimerizes to form a hexameric cellular signaling complex (1, 4). Human IL-6Ra consists of an extracellular domain (ECD) with an N-terminal Ig-like C2-type domain and a cytokine-binding domain containing two fibronectin type-III domains with a WSXWS motif, a helical transmembrane segment and a cytoplasmic domain. Within the mature ECD, human IL-6Ra shares 51% and 52% amino acid sequence identity with mouse and rat IL-6Ra, respectively. Soluble forms of IL-6Ra can be generated via ADAM10 and ADAM17 cleavage of membrane bound IL-6Ra, alternative mRNA splicing and microvesicle release (3, 4). Unlike gp130 that is expressed ubiquitously, the cellular distribution of IL-6 R alpha is predominantly limited to hepatocytes and leukocyte subpopulations such as monocytes, neutrophils, T and B cells. Soluble IL-6R alpha has been found in various body fluids (5). It has been documented that elevated soluble IL-6 R is associated with numerous diseases including arthritic lesions, multiple myeloma and Crohn's disease (6, 7). Our Avi-tag Biotinylated IL-6Ra features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.
Source : CHO cells
Species : Human
Tag : His/Avi
Predicted N Terminal : Leu20
Form : Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Bio-activity : Measured by its binding ability in a functional ELISA. When Human IL6 is immobilized at 0.25 µg/mL (100 µL/ well), Biotinylated Recombinant Human IL6R His-tag Avi‑tag binds with an ED50 of 0.1-1 μg/mL.
Molecular Mass : 65-80 kDa, under reducing conditions
Protein length : Leu20-Pro365
Endotoxin : <0.10 EU per 1 μg of the protein by the LAL method.
Purity : >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Applications : Bioactivity
Storage : Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 °C as supplied.1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution : Reconstitute at 1 mg/mL in PBS.
Gene Name : IL6R interleukin 6 receptor [ Homo sapiens ]
Official Symbol : IL6R
Synonyms : IL6R; interleukin 6 receptor; interleukin-6 receptor subunit alpha; CD126; IL-6R 1; CD126 antigen; membrane glycoprotein 80; IL-6 receptor subunit alpha; gp80; IL6RA; IL-6RA; IL-6R-1; MGC104991;
Gene ID : 3570
mRNA Refseq : NM_000565
Protein Refseq : NP_000556
MIM : 147880
UniProt ID : P08887

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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What is the dose and route of administration of IL6R? 04/30/2022

The dose and route of administration need to be determined according to the specific treatment situation. Common routes of administration include subcutaneous injection and intravenous injection.

Can recombinant IL6R be used in autoimmune diseases? 04/03/2022

It can be used to treat certain autoimmune diseases, such as rheumatoid arthritis and psoriasis.

Can recombinant IL6R be used to prevent disease? 09/14/2021

The IL6R is primarily used to treat inflammatory diseases that have already occurred, rather than prevent them.

How the action mechanism of IL6R? 03/20/2021

IL6R reduces inflammation by binding to interleukin-6 (IL-6), blocking the IL-6 signaling pathway.

Does IL6R have anti-tumor effects? 11/06/2020

Although IL6R can regulate the immune system, it is not a direct anti-tumor drug.

How to evaluate the efficacy of IL6R? 06/29/2019

The efficacy of IL6R is usually evaluated based on improvement of clinical symptoms, changes in disease activity, and changes in biochemical markers.

Customer Reviews (3)

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Reviews
02/23/2022

    Good compatibility and stability with the surfactant.

    11/23/2020

      Expression level and stability of clones were good.

      02/01/2019

        Remarkable antibacterial activity and bactericidal effect on bacteria.

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