Active Recombinant Human Klotho, His-tagged

• Cat.No.: KL-388H
• Product Overview: Recombinant Human KL(Glu34-Ser981), fused with a C-terminal 6-His tag, was expressed in Mouse NSO Cells.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: His
• Protein Length: 34-981 a.a.
• Description: This gene encodes a type-I membrane protein that is related to beta-glucosidases. Reduced production of this protein has been observed in patients with chronic renal failure (CRF), and this may be one of the factors underlying the degenerative processes (e.g., arteriosclerosis, osteoporosis, and skin atrophy) seen in CRF. Also, mutations within this protein have been associated with ageing and bone loss.
• Predicted N Terminal: Glu34
• Form: Supplied as a 0.2 µm filtered solution in MES, Glycerol and EDTA.
• Bio-activity: Measured in a cell proliferation assay using NIH-3T3 mouse embryonic fibroblast cells. The ED50 for this effect is typically 0.025 - 0.1 µg/mL in the presence of 1 µg/mL of rhFGF-23 and 10 µg/mL of Heparin.Measured in a cell proliferation assay using BaF3 mouse pro-B cells transfected with human FGF RIIIc. The ED50 for this effect is typically 0.025 - 0.1 µg/mL in the presence of rhFGF-23 and Heparin.
• Molecular Mass: Predicted Molecular Mass: 109.8 kDaSDS-PAGE: 120-130 kDa, reducing conditions
• Endotoxin: < 1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles.12 months from date of receipt, -70 °C as supplied.1 month, 2 to 8 °C under sterile conditions after opening.

Gene Information

• Gene Name: KL klotho [ Homo sapiens (human) ]
• Official Symbol: KL
• Synonyms: KL; klotho; NP_004786.2; EC 3.2.1.31
• Gene ID: 9365
• mRNA Refseq: NM_004795
• Protein Refseq: NP_004786
• MIM: 604824
• UniProt ID: Q9UEF7
• Chromosome Location: 13q12
• Pathway: Adaptive Immune System; DAP12 interactions; Downstream signaling events of B Cell Receptor (BCR)
• Function: beta-glucosidase activity; fibroblast growth factor binding; fibroblast growth factor receptor binding