Active Recombinant Human Laminin α4

• Cat.No.: LAMA4-6952H
• Product Overview: Recombinant Human Lamininα4 protein, was expressed in Chinese Hamster Ovary cell line, CHOderived andpurified by SDS-PAGE

Specification

• Species: Human
• Source: CHO
• Tag: Non
• Description: Laminin subunitalpha-4 is a protein that in humans is encoded by the LAMA4 gene. Laminins, afamily of extracellular matrix glycoproteins, are the major noncollagenousconstituent of basement membranes. They have been implicated in a widevariety of biological processes including cell adhesion, differentiation,migration, signaling, neurite outgrowth and metastasis. Laminins are composedof 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, andB2, respectively) and they form a cruciform structure consisting of 3 short arms,each formed by a different chain, and a long arm composed of all 3 chains.Each laminin chain is a multidomain protein encoded by a distinct gene.Several isoforms of each chain have been described
• MolecularMass: 111 kDa
• Purity: >90%, by SDSPAGE underreducing conditions and visualized by silver stain.
• Activity: Measured by theability of the immobilized protein to support the adhesion of HT1080 humanfibrosarcoma cells. The ED50 for this effect is typically 0.7-2.8 μg/mL.Optimal dilutions should be determined by each laboratory for eachapplication
• EndotoxinLevel: <1.0 EU per 1 μgof the protein by the LAL method.
• N-terminalSequenceAnalysis: Gln826 predicted,sequencing might be blocked, no result obtained
• Formulation: Lyophilized from a0.2 μm filtered solution in PBS.
• Reconstitution: Reconstitute at 200μg/mL in PBS
• Storage: Use a manual defrostfreezer and avoid repeated freeze thaw Cycles. 12 months from date ofreceipt, -20 to -70°C as supplied. 1 month, 2 to 8 °C under sterileconditions after reconstitution. 3 months, -20 to -70°C under sterileconditions after reconstitution.

Gene Information

• Gene Name: LAMA4 laminin, alpha 4 [ Homosapiens ]
• Official Symbol: LAMA4
• Synonyms: LAMA4;laminin, alpha 4; LAMA3; LAMA4*-1; DKFZp686D23145; Laminin-14 subunit alpha;Laminin-8 subunit alpha; Laminin-9 subunit alpha
• Gene ID: 3910
• mRNA Refseq: NM_001105206
• Protein Refseq: NP_001098676
• MIM: 600133
• UniProt ID: Q16363
• Chromosome Location: 6q21
• Pathway: extracellularmatrix structural constituent; receptor binding
• Function: Africantrypanosomiasis; Amoebiasis; ECM-receptor interaction; FOXM1 transcriptionfactor networ; Focal adhesion; Pathways in cancer; Small cell lung cancer;Toxoplasmosis