Active Recombinant Human Matrilin 3, His-tagged

• Cat.No.: MATN3-5685H
• Product Overview: Recombinant Human MATN3protein was expressed in Murine myeloma cell line with a C-terminal 6-Histag. Asp29-Arg486.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: His
• Protein Length: 29-486 a.a.
• Description: MATN3 is a 50-60 kDaextracellular matrix protein that belongs to the superfamily of vonWillebrand Factor A containing proteins. It is primarily expressed incartilage and functions as a bridging component between proteins of thecollagenous matrix. The human MATN3 cDNA encodes a 486 amino acid (aa)precursor with a 28 aa signal sequence, an N-terminal VWA domain, four tandemEGF-like repeats, and a C-terminal coiled coil domain. The Matrilins differin the number of VWA domains and EGF-like repeats they contain. Human MATN3 shares82% aa sequence identity with mouse MATN3.
• Form: Lyophilized from a0.2 μm filtered solution in PBS.
• N-terminalSequence: Asp29
• Molecular Weight: 50.6 kDa
• Activity: Measuredby its binding ability in a functional ELISA. Immobilized rhCOMP at 2 μg/mL(100 μL/well) can bind rhMATN3 with alinear range of 1.5-100 ng/mL.
• Endotoxin Level: <1.0 EUper 1 μg of the protein by the LAL method.
• SDS-PAGE: 57 kDa,reducing conditions.
• Purity: >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: 100μg/mL in sterile PBS
• Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months fromdate of receipt, -20 to -70°C as supplied. 1 month, 2 to 8°C under sterileconditions after reconstitution. 3 months, -20 to -70°C under sterileconditions after reconstitution.

Gene Information

• Gene Name: MATN3 matrilin 3 [ Homosapiens ]
• Official Symbol: MATN3
• Synonyms: MATN3; matrilin 3; HOA;OS2; EDM5; DIPOA; OADIP; matrilin-3; OTTHUMP00000200547
• Gene ID: 4148
• mRNA Refseq: NM_002381
• Protein Refseq: NP_002372
• MIM: 602109
• UniProt ID: O15232
• Chromosome Location: 2p24-p23
• Function: calcium ion binding;extracellular matrix structural constituent; protein binding