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Active Recombinant Human OTUD3, GST-tagged

Cat.No. : OTUD3-178H
Product Overview : Recombinant human OTUD3 (amino acid residues 1-629), fused with N-terminal GST, was expressed in E.coli.
  • Specification
  • Gene Information
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Description : There are two main classes of DUB, cysteine proteases and metalloproteases. OTUD3 is a cysteine protease and is a member of the OTU superfamily of proteins (Balakirev et al., 2003). Cloning of the human gene was first described by Seki et al. (1999). OTU enzymes play important roles as negative-feedback regulators in NF-κB signalling, interferon signalling and in p97 (cdc48)-mediated processes although the cellular functions of most OTU enzymes remain to be discovered.
Source : E. coli
Species : Human
Tag : GST
Form : 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol
Bio-activity : Deubiquitylase Enzyme Assay: The activity of GST-OTUD3 was validated by determining the increase in fluorescence measured as a result of the enzyme catalysed cleavage of the fluorogenic substrate Ubiquitin-Rhodamine110-Glycine generating Ubiquitin and Rhodamine110-Glycine. Incubation of the substrate in the presence or absence of GST-OTUD3 was compared confirming the deubiquitylating activity of GST-OTUD3.
Molecular Mass : ~72 kDa
Purity : >98% by SDS-PAGE
Storage : 12 months at -70°C. Avoid multiple freeze/thaw cycles.
Concentration : 0.5 mg/ml
Gene Name : OTUD3 OTU domain containing 3 [ Homo sapiens ]
Official Symbol : OTUD3
Synonyms : OTUD3; OTU domain containing 3; OTU domain-containing protein 3; DUBA4; KIAA0459; RP11-460G22.1;
Gene ID : 23252
mRNA Refseq : NM_015207
Protein Refseq : NP_056022
MIM : 611758
UniProt ID : Q5T2D3
Chromosome Location : 1p36.13

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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