| Description : |
Human Platelet-derived growth factor -BB Platelet-derived growth factor (PDGF) presenting in serum but absent from plasma was first discovered in animal study by Lynch and co-workers in the late 1980s. It is a disulfide-linked dimer consisting of two peptides-chain A and chain B. PDGF has three subforms: PDGF-AA, PDGF-BB, PDGF-AB. It is involved in a number of biological processes, including hyperplasia, embryonic neuron development, chemotaxis, and respiratory tubule epithelial cell development. The function of PDGF is mediated by two receptors (PDGFR-α and PDGFR-β). |
| Source : |
E. coli |
| Species : |
Human |
| Form : |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Bio-activity : |
Fully biologically active when compared to standard.The ED50 as determined by the dose-dependent stimulation of the proliferation of murine Balb/c 3T3 cells is ≤ 3.0 ng/mL, corresponding to a specific activity of ≥ 3.3 × 10^5 units/mg. |
| Molecular Mass : |
Approximately 12.4 KDa, a disulfide-linked homodimeric protein containing two 109 amino acid residues polypeptide (B chain). |
| Protein Length : |
109 |
| AA Sequence : |
SLGSLTIAEPAMIAECKTRTEVFEI SRRLIDRTNANFLVWPPCVEVQRCS GCCNNRNVQCRPTQVQLRPVQVRKI EIVRKKPIFKKATVTLEDHLACKCE TVAAARPVT |
| Endotoxin : |
Less than 1 EU/μg of rHuPDGF-BB as determined by LAL method. |
| Purity : |
>97% by SDS-PAGE and HPLC analyses. |
| Storage : |
This lyophilized preparation is stable at 2-8 centigrade, but should be kept at -20 centigrade for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 centigrade. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 to -70 centigrade. Avoid repeated freeze/thaw cycles. |
| Storage Buffer : |
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Reconstitution : |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20C. Further dilutions should be made in appropriate buffered solutions. |
