Active Recombinant Mouse FCGR3 protein, His-tagged

• Cat.No.: FCGR3-1457M
• Product Overview: Recombinant Mouse FCGR3 protein(Leu32-Thr215), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Mouse
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4.
• Bio-activity: Loaded Mouse CD16, His Tag on NTA Biosensor, can bind OKT3 with an affinity constant of 0.78 μM as determined in BLI assay (ForteBio Octet Red96e).Loaded Mouse CD16, His Tag on NTA Biosensor, can bind Herceptin with an affinity constant of 2.8 μM as determined in BLI assay (ForteBio Octet Red96e).
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 23.0 kDa. The protein migrates as 36-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Leu32-Thr215
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE. >90% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Protein length: Leu32-Thr215

Gene Information

• Gene Name: Fcgr3 Fc receptor, IgG, low affinity III [ Mus musculus ]
• Official Symbol: FCGR3
• Synonyms: FCGR3; Fc receptor, IgG, low affinity III; low affinity immunoglobulin gamma Fc region receptor III; fcRIII; FcgammaRIII; fc-gamma RIII; Fcg receptor III; igG Fc receptor III; Fc gamma receptor III; CD16
• Gene ID: 14131
• mRNA Refseq: NM_010188
• Protein Refseq: NP_034318