| Species : |
Streptococcus pneumoniae |
| Source : |
E. coli |
| Tag : |
Non |
| Description : |
Sialidase Sp cleaves the non-reducing terminal α(2-3) unbranched sialic acid residues from complex |
| Form : |
Sterile-filtered solution |
| EC : |
3.2.1.18 |
| Specificity : |
Cleaves the non-reducing terminal α(2-3) unbranched sialic acid residues from complex carbohydrates and glycoproteins. |
| Contents : |
Sialidase Sp in 50 mM sodium phosphate, pH 7.5
5× Reaction Buffer 250 mM sodium phosphate, pH 6.0 |
| Bio-activity : |
Activity: 10 U/mL
Specific Activity: 250 U/mg
Defined as the amount of enzyme required to produce 1 μmole of methylumbelliferone in 1 minute at 37 centigrade, pH 5.0 from MU-NANA [2′-(4-methylumbelliferyl)-α-D-N-acetylneuraminic acid]. |
| Molecular Mass : |
~75 kDa |
| Suggested usage : |
1. Add up to 100 μg of glycoprotein or 1 nmol of oligosaccharide to tube.
2. Add water to 14 μL
3. Add 4 μL 5× Reaction Buffer.
4. Add 2 μL α(2-3) Sialidase Sp.
5. Incubate at 37 centigrade for 1 hour
Desialylation may be monitored by SDS-PAGE if the size differential between native and desialylated protein is sufficient for detection. |
| Unit Definition : |
One unit of Neuraminidase is defined as the amount of enzyme required to produce 1 μmole of methylumbelliferone in 1 minute at 37 centigrade, pH 5.0 from MU-NANA (2'.-(4-methyl-umbelliferyl)-alpha-D-N acetylneuraminic acid]. |
| pH optimum : |
6 |
| Purity : |
Neuraminidase Sp is tested for contaminating protease as follows: 10 μg of denatured BSA is incubated at 37 centigrade for 24 hours with 2 μL of enzyme. SDS-PAGE analysis of the treated BSA shows no evidence of degradation.
The production host strain has been extensively tested and does not produce any detectable glycosidases. |
| Applications : |
1. Structural analysis of oligosaccharides
2. Determining sialic acid linkage
3. Glycoprotein deglycosylation
4. Removing heterogeneity from glycoproteins |
| Stability : |
Stable at least 12 months when stored properly. Several days exposure to ambient temperatures will not reduce activity. |
| Storage : |
Store enzyme at 4 centigrade. |
| Storage Buffer : |
The enzyme is provided as a sterile-filtered solution in 50 mM Sodium phosphate pH 7.5. |
