AK Activity Assay kit can kinetically measure Adenylate Kinase activity by detecting adenosine triphosphate (ATP) generated from adenosine diphosphate (ADP) as a substrate. ATP is detected via a multi-step reaction, resulting in the generation of an intermediate that reacts with the Adenylate Kinase Probe forming an end product that can be measured colorimetrically (OD 570 nm) or fluorometrically (Ex/Em = 535/587 nm).
Size :
100 assays
Applications :
Detection of Adenylate Kinase activity
Storage :
Store kit at -20°C, protected from light. Briefly centrifuge small vials prior to opening. Read entire protocol before performing the assay.AK Assay Buffer: Bring to room temperature before use. Store at -20°C or 4°C.AK Convertor and AK Developer: Reconstitute each with 220 µl AK Assay Buffer and mix gently by pipetting. Briefly centrifuge to collect the contents at the bottom of the tube. Aliquot and store at -20°C. Avoid repeated freeze/thaw.Positive Control (AK Enzyme): Reconstitute with 55 µl deionized water. Store at -20°C. Avoid repeated freeze/thaw. Use within two months.ATP Standard: Dissolve in 100 µl of distilled water to generate a 10 mM stock solution. Keep on ice while in use. Store at -20°C. Avoid repeated freeze/thaw.
