"ATPase" Related Products

ATPase Activity Assay Kit (Colorimetric)

Cat.No.: Kit-1031
Description: ATPase (Adenosine Triphosphatase: EC 3.6.1.3) is an important enzyme for maintaining the cell membrane potential, transporting ions and regulating cellular volume. It catalyzes the decomposition of ATP into ADP and a free phosphate ion. The hydrolysis of ATP is highly exergonic releasing energy that is utilized in several cellular processes. There are many classes of ATPases including Na+/K+ -ATPase, H+/K+ -ATPase, Ca2+ -ATPase, etc. The deficiency of mitochondrial ATPase is serious: for example, Na+/K+-ATPase deficiency increases anxiety-related behavior, while Ca2+ -ATPase deficiency leads to exertional muscle pain syndrome. Therefore, accurate detection of ATPase activity is valuable for the diagnosis and mechanistic studies of some of these diseases. ATPase Activity Assay kit provides a quick and easy method for monitoring ATPase activity in various samples. In the assay, ATPase hydrolyzes ATP releasing ADP and a free phosphate ion, and through linked reactions, a strong, stable chromophore is generated (OD 650 nm). The assay is simple, sensitive, high -throughput adaptable and can detect ATPase Activity less than 0.005 U/L.
Applications: Rapid assessment of ATPase activity in biological samples or recombinant ATPase preparations
The assay is designed to measure ATPase activity in biological samples with detection sensitivity ~5 mU/l
Storage: -20°C
Shipping: Gel Pack
Size: 100 assays
Kit Components: ATPase Assay Buffer; ATPase Substrate; ATPase Developer; Phosphate Standard (10 mM); ATPase Positive Control
Target Species: Mammalian
Detection method: Colorimetric (OD 650 nm)
Features & Benefits: Simple procedure; takes less than 1 hour; Fast and convenient

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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