Measurement of ATPase activity in various tissues/cells Analysis of energy-generating pathways Analysis of Na+/K+ -ATPase mediated signal transduction pathways, e.g. MAPK, ROS etc.
Store kit at -20ºC, protected from light. Centrifuge vials briefly, prior to use. ATPase Assay Buffer: Warm to room temperature before use. Store at 4ºC. ATPase Substrate: Reconstitute one well with 110 µl dH2O. Pipette up and down to dissolve. Aliquot and store at -20°C. Use within two months. ATPase Developer: Ready to use as supplied and keep it at room temperature. ATPase Positive Control: Reconstitute with 100 μl Assay Buffer and mix thoroughly. Keep on ice while in use. Aliquot and store at –20°C. Use within two months.