Caspase-8 assay Kit for drug discovery


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Caspase-8 assay Kit for drug discovery

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Cat.No. : Kit-0172
Product Overview : The CASPASE-8 Drug Discovery Kit is a complete assay system designed to screen caspase-8 inhibitors. It contains both a colorimetric substrate (IETD-pNA) and a fluorogenic substrate (IETD-AMC). Cleavage of the p-nitroanilide (pNA) from the colorimetric substrate increases absorption at 405nm. The fluorescent assay is based on the cleavage of 7-amino-4-methylcoumarin (AMC) dye from the C-terminus of the peptide substrate. Cleavage of the dye from the substrate increases its fluorescence intensity at 460 nm. The assays are performed in a convenient, 96-well microtiter-plate format. The kit is useful to screen inhibitors of caspase-8, a potential therapeutic target. An inhibitor, IETD-CHO (aldehyde), is included for use as a control.
Size : 96 wells
Storage : Store all components except the microplate at -70°C for the highest stability. The caspase-8 enzyme component must be handled particularly carefully in order to retain maximal enzymatic activity. Defrost it quickly in a RT water bath or by rubbing between fingers, then immediately store on an ice bath. The remaining unused enzyme should be quickly refrozen by placing at -70°C. The enzyme is stable to freeze/thaw cycles x 4. To minimize the number of freeze/thaw cycles, aliquot the caspase-8 into separate tubes and store at -70°C.

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