CETP Activity Fluorometric Assay Kit II


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CETP Activity Fluorometric Assay Kit II

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Cat.No. : Kit-0209
Product Overview : CETP Activity Assay Kit II (Fluorometric) uses a donor molecule containing a fluorescent self-quenched phospholipid that is transferred to an acceptor molecule in the presence of Cholesteryl Ester Transfer Protein (CETP). CETP-mediated transfer of the fluorescent phospholipid to the acceptor molecule results in an increase in fluorescence (Ex/Em = 480/511 nm). The fluorometric intensity is directly proportional to the amount of phospholipid transferred. The kit contains rabbit serum and torcetrapib (CETP inhibitor) as positive and negative control, respectively, for assay validation.This assay can measure PLTP activity in plasma and serum but can also be used for testing activity of recombinant CETP protein.
Applications : Functional Studies
Storage : Store kit at 4ºC in the dark immediately upon receipt, except fromthe CETP Positive Control which should be stored at -20°C. Kithas a storage time of 1 year from receipt, providing componentshave not been reconstituted.Refer to list of materials supplied for storage conditions of individualcomponents. Observe the storage conditions for individual preparedcomponents in section 5.Aliquot components in working volumes before storing at therecommended temperature. Reconstituted components are Stablefor 2 months.

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