Cholinesterase Activity Assay Kit (Colorimetric)
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|Applications :||Measurement of ChE activity in various tissues/cells
Screening of ChE inhibitors
|Storage :||Store Kit at -20°C, protect from light. Briefly centrifuge small vials prior to opening. Read entire protocol before performing the assay.
ChE Assay Buffer: Store at 4 ºC or -20 ºC. Bring to room temperature before use.
AChE Substrate: Reconstitute in 100 µl ChE Assay Buffer. Store at -20ºC. Use within two months.
BChE Substrate: Store at -20°C, protect from light. Bring to room temperature before use.
Acetylcholinesterase: Reconstitute with 100 µl of ChE Assay Buffer. Aliquot and store at -20 ºC. Use within two months.
Butyrylcholinesterase: Reconstitute with 20 µl ChE Assay Buffer. Store at -20 ºC. Use within two months.
BChE Inhibitor: Reconstitute BChE Inhibitor in 150 µl dH2O. Vortex intensively at room temperature to facilitate solubilization. Aliquot and store at -20ºC. Bring to room temperature before use. Use it within two months.
DTNB Solution: Dissolve 1 vial of DTNB with 625 µl ChE Assay Buffer. Each vial can be used to carry out up to 50 reactions. Dissolve
vial contents when needed. Store at -20ºC. Use within two months.
TNB Standard: Dissolve in 1 ml of ChE Assay Buffer to generate 2.5 mM TNB Standard. Use within two months.
|Size :||100 assays|