Con A Agarose

Cat.No. : Con A-001A
Product Overview : Con A agarose is an affinity medium with concanavalin A (Con A) coupled to agarose 4B by the cyanogen bromide method, which is a?chromatography?medium?for?separation?and?purification?of?glycoproteins,?polysaccharides,?and?glycolipids.
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Characteristics : ConA binds specifically α-D-mannosyl and α-D-glucosyl residues in terminal position of ramified structures from B-Glycans. It has 4 binding sites, corresponding to the 4 sub-units. The molecular weight is 104-112KDa and the isoelectric point (pI) is in the range of 4.5-5.5. The binding sugar requires the presence of C-3, C-4 and C-5 hydroxyl groups for reaction with Con A. Con A coupled to agarose is routinely used for separation and purification of glycoproteins, polysaccharides and glycolipids.
Size : 5 mL, 100 mL
Matrix : 4% agarose
Functional group : Con A
Average particle size : 90μm
Ligand : Con A
Ligand concentration : 10?to?15?mg?Con?A/ml?medium
Dynamic binding capacity : 20-45?mg?porcine?thyroglobulin/ml?medium
Recommended flow rate : 75 cm/h
Recommended column height : 5‐20cm
Chemical stability : Stable?in?all?commonly?used?aqueous?buffers.?Chelating?agents?such?as?EDTA,?8?M?urea,?or?solutions?having?pH?below?3?should?be?avoided?as?these?conditions?result?in?removal?of?manganese?from?the?lectin?with?loss?of?activity as a result.
pH working range : 4-9
Warning : Avoid using Chelating agents such as EDTA, 8 M urea or solutions having a pH below 3 should be avoided as these conditions results in removal of manganese from the lectin with loss of activity as a result.

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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.


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