Continuous AMP Deaminase Assay Kit

• Cat.No.: Kit-0875
• Product Overview: AMPD Assay Kit is the first non-radioactive and continuous kit designed to measure AMP deaminase content in samples. This enzymatic assay is based on a reaction involving Inosine Monophosphate Dehydrogenase (IMPDH).
  The principle of the assay is based on the coupling of the following enzymatic reactions:
  (1) In the presence of AMP, AMP Deaminase enzyme catalyzes the formation of IMP.
  (2) In the presence of NAD, IMP is immediately oxidized by a highly active IMPDH in the presence of NAD with simultaneous formation of NADH2 directly monitored spectrophotometrically at 340 nm.
  The assay is developed for measuring AMPDA activity in vitro or in cell lysates.
  For maximal accuracy, the assays with cell lysates are run with and without AMP in parallel. The absorbance rate observed in the absence of AMP is used as blank and is subtracted from the absorbance rate measured in its presence.

Specification

• Storage: Once dissolved, the reagents provided in the kit are not stable and should be stored on ice and used the day of preparation.
• Size: 24 analysis
• Publication:
  Lactate metabolism is essential in early-onset mitochondrial myopathy (2023)

Reviews

11/11/2021

This protein exhibits exceptional purity and reliability, ensuring accurate and reproducible results in my assays.

11/10/2019

I am extremely pleased with the quality of the AMPD protein as it perfectly fulfills my experimental requirements.

04/14/2017

I have full confidence in the AMPD protein and the exceptional support provided by the manufacturer, making it my top recommendation for researchers seeking a high-quality protein for their experimental needs.

12/15/2016

Their knowledgeable team has been readily available to address any concerns or queries, providing effective solutions and guidance whenever needed.

Q&As

How is the AMPD protein regulated?

The activity of the AMPD protein can be regulated by several factors, including allosteric regulation and post-translational modifications. Allosteric regulation occurs when certain molecules bind to the enzyme and modify its activity.

Is the AMPD protein a target for therapeutic interventions?

The AMPD protein is not currently a widely targeted therapeutic option. However, its dysregulation in certain disease conditions, such as cardiac dysfunction, has sparked interest in exploring its potential as a therapeutic target. Modulating AMPD activity or its downstream effects may hold promise for managing certain health conditions, but further research is needed to understand its full therapeutic potential.

Are there any genetic variants or mutations associated with the AMPD protein?

Yes, genetic variants in the AMPD1 gene, which codes for the AMPD protein, have been identified. Some variants, such as the common c.34C>T variant, can result in decreased enzyme activity and have been associated with increased risk of developing certain health conditions, including exercise-induced myopathy and cardiac dysfunction.

Are there any lifestyle modifications or management strategies for individuals with AMPD protein deficiency?

For individuals with AMPD protein deficiency, certain lifestyle modifications and management strategies may be beneficial. These can include avoiding strenuous exercise or activities that trigger muscle symptoms, maintaining a balanced diet, and managing stress levels. It is important to consult with a healthcare professional or genetic counselor to develop an appropriate management plan based on individual circumstances.

Are there any diagnostic tests for AMPD protein deficiency?

Yes, there are diagnostic tests available to assess AMPD protein deficiency. One common method is to measure the activity of the enzyme in a blood sample. If the enzyme activity is significantly decreased, it may suggest an AMPD protein deficiency. Genetic testing can also be performed to identify specific variants in the AMPD1 gene associated with the deficiency.

Is there ongoing research on the AMPD protein and its implications?

Yes, there is ongoing research on the AMPD protein and its implications in various health conditions. Scientists are studying the underlying mechanisms of AMPD protein function, regulation, and its relationship with cellular processes. Furthermore, research is focused on understanding the impact of genetic variants in the AMPD1 gene and their association with disease risk and progression. This research aims to pave the way for potential therapeutic interventions and improved management strategies in the future.

Are there any diseases or conditions associated with abnormalities in the AMPD protein?

Variants in the AMPD1 gene have been linked to various health conditions. For example, mutations that lead to reduced AMPD activity can result in exercise-induced muscle symptoms, such as muscle pain, fatigue, and cramps. Additionally, alterations in AMPD activity have been associated with cardiac dysfunction, including an increased risk of heart failure and adverse cardiovascular outcomes.

Can AMPD protein supplementation be considered as a treatment?

Currently, there is limited evidence on the use of AMPD protein supplementation as a treatment for AMPD protein deficiency or associated conditions. While it may seem logical to supplement with the missing enzyme, the practicality and effectiveness of such supplementation are not well-established. Further research is needed to explore the potential benefits and safety of AMPD protein supplementation.

Can AMPD protein deficiency lead to any health problems?

Yes, AMPD protein deficiency, caused by genetic variants that result in decreased enzyme activity, can lead to certain health problems. One of the most common manifestations is exercise-induced muscle symptoms, also known as exercise intolerance myopathy. This can include muscle pain, weakness, cramps, and fatigue during physical activity. Additionally, cardiac dysfunction, such as an increased risk of heart failure, has been associated with AMPD protein deficiency.