Continuous PRPP-S Assay Kit Kit-0871

Specification

Cat.No. :	Kit-0871
Product Overview :	PRPP-S Assay Kit is designed to measure PRPP (α-D-5-phosphoribosyl-1-pyrophosphate) content in samples. This enzymatic assay is based on a coupled reaction involving Hypoxanthine-guanine phosphoribosyltransferase (HGPRT) and Inosine Monophosphate Dehydrogenase (IMPDH). The principle of the assay is based on the coupling of the following enzymatic reactions: (1) In the presence of ATP and P-ribose, PRPP-Synthetase enzyme catalyzes the formation of PRPP. (2) In the presence of Hypoxanthine (Hx), PRPP is converted to IMP by Hypoxanthine-guanine phosphoribosyltransferase (HGPRT). (3) IMP is immediately oxidized by a highly active IMPDH in the presence of NAD with simultaneous formation of NADH2 directly monitored spectrophotometrically at 340nm. The assay is developed for measuring PRPP-S activity in vitro or in cell lysates. For maximal accuracy, the assays with cell lysates are run with and without P-ribose in parallel. The absorbance rate observed in the absence of inosine is used as blank and is subtracted from the absorbance rate measured in its presence.
Storage :	Once dissolved, the reagents provided in the kit are not stable and should be stored on ice and used the day of preparation.
Size :	24 analysis

PRODUCTS