Recombinant E. coli Eco, His-tagged

• Cat.No.: eco-3423E
• Product Overview: Recombinant E. coli Ecotin protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Species: E.coli
• Source: E.coli
• Tag: His
• Description: Ecotin (E. coli serine protease inhibitor) inhibits pancreatic serine proteases. This protein inhibits chymotrypsin, trypsin, elastases, factor X, kallikrein as well as a variety of other proteases. The strength of inhibition does not appear to be correlated with particular protease specificity.
• Form: Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 50mM NaCl, 1mM DTT, 10% glycerol.
• Molecular Weight: 18.3 kDa (163aa) confirmed by MALDI-TOF.
• Purity: > 95% by SDS - PAGE.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MAESVQPLEK IAPYPQAEKG MKRQVIQLTP QED ESTLKVE LLIGQTLEVD CNLHRLGGKL ENKTLEGWGY DYYVFDKVSS PVS TM MACPD GKKEKKFVTA YLGDAGMLRY NSKLPIVVYT PDNVDVKYRV WK AEE KIDNA VVR.
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: eco ecotin, a serine protease inhibitor [ Escherichia coli str. K-12 substr. MG1655 ]
• Synonyms: ecotin, a serine protease inhibitor; ECK2201; eti; JW2197; eco;ecotin, a serine protease inhibitor
• Gene ID: 946700
• Protein Refseq: NP_416713

Citation

CDC25B partners with PP2A to induce AMPK activation and tumor suppression in triple negative breast cancer

Journal: NAR Cancer    PubMed ID: 33385163    Data: 2020/12/1

Authors: Junmei Cairns, Reynold C Ly, Liewei Wang

Article Snippet:Recombinant Human CDC25B and CDC25A protein, fused to GST-tag, was expressed in Escherichia coli and purified by GSH-sepharose.Recombinant Human CDC25B and CDC25A protein, fused to GST-tag, was expressed in Escherichia coli and purified by GSH-sepharose.. Recombinant Human PP2A-A, PP2A-B and PP2A-C, fused with a polyhistide tag at the N-terminus, was produced in E. coli . (Creative BioMart).. The GST-CDC25B plasmids were transformed into E. coli cells.The GST-CDC25B plasmids were transformed into E. coli cells.

Cytosolic PCNA interacts with p47phox and controls NADPH oxidase NOX2 activation in neutrophils

Journal: The Journal of Experimental Medicine    PubMed ID: 31492810    Data: 2019/11/4

Authors: Delphine Ohayon, Alessia De Chiara, Véronique Witko-Sarsat

Article Snippet:Recombinant human p47phox, p67phox, and Rac2 were produced as previously described ( ).Recombinant human p47phox, p67phox, and Rac2 were produced as previously described ( ).. Recombinant p40phox expressed in E. coli was purchased from Creative BioMart.. To generate the PX domain of p47phox, a truncated p47phox (residues 2–132) was produced in E. coli using the pGEX plasmid (generous gift of Dr. Yaffe, Massachusetts Institute of Technology, Cambridge, MA).To generate the PX domain of p47phox, a truncated p47phox (residues 2–132) was produced in E. coli using the pGEX plasmid (generous gift of Dr. Yaffe, Massachusetts Institute of Technology, Cambridge, MA).

Significance of filamin A in mTORC2 function in glioblastoma

Journal: Molecular Cancer    PubMed ID: 26134617    Data: 2015/7/2

Authors: Naphat Chantaravisoot, Piriya Wongkongkathep, Fuyuhiko Tamanoi

Article Snippet:Anti-AU1 agarose beads were from BETHYL Lab. AU1 peptide (DTYRYI) was from COVANCE.Anti-AU1 agarose beads were from BETHYL Lab. AU1 peptide (DTYRYI) was from COVANCE.. Recombinant C-terminal fragment (amino acids 1730–2639) of Human FLNA purified from E. coli was obtained from Creative BioMart.. Full-length inactive recombinant AKT1/PKB was from EMD Millipore.Full-length inactive recombinant AKT1/PKB was from EMD Millipore.