||Recombinant human LIPG / EDL (rhLIPG) Ser21-Pro500 fused with a N-terminal his tag was produced in human HEK293 cells.
||Lipase endothelial (LIPG), also known as Endothelial lipase (EL/EDL). This protein has substantial phospholipase activity and may be involved in lipoprotein metabolism and vascular biology. LIPG is designated a member of the triglyceride (TG) lipase family by its sequence and characteristic lid region which provides substrate specificity for enzymes of the TG lipase family. LIPG mediates both HDL binding and uptake,and the selective uptake of HDL-CE, independently of lipolysis and CLA-1. LIPG also mediates binding and uptake of plasma lipoproteins via a process that is independent of its enzymatic activity, requires cellular heparan sulfate proteoglycans, and is regulated by ligand clustering.LIPG may have unique functional roles in the pathogenesis of coronary artery diseases such as atherosclerosis as well as in lipid metabolism in the vessel wall.
||>92% as determined by SDS-PAGE.
||Less than 1.0 EU per 1 μg of the rhLIPG by the LAL method.
||55.4 kDa. Protein migrates as 55-65 kDa in reduced SDS-PAGE resulting from glycosylation.
||Supplied as a 0.22 μm filtered solution in PBS, pH 7.4 and delivered as liquid formulation or lyophilized powder. Normally mannitol or trehalose are added as protectants before lyophilization.
||No activity loss was observed after storage at 4-8°C for 1 month or -20°C for 1 year in lyophilized state. rhLIPG should be aliquoted and stored at -80°C in liquid formulation, for long term storage. Avoid repeated freeze-thaw cycles.