Species : |
Human |
Source : |
CHO |
Tag : |
His |
Protein Length : |
23-1321 a.a. |
Description : |
NCAN, also known asCSPG3 and 1D1, is a secreted chondroitin sulfate proteoglycan that isprimarily expressed in the central nervous system. Human NCAN contains oneIg-like V-type domain, two Link domains, two EGF-like domains, one C-typelectin like domain, and one Sushi domain. It is an approximately 300 kDamolecule of which 66 kDa is chondroitin sulfate and 60 kDa is Nand O-linked glycosylation.Mature human NCAN shares 66% aa sequence identity with mature mouse and ratNCAN. |
Form : |
Lyophilized from a0.2 μm filtered solution in PBS. |
N-terminalSequence : |
Glu23 |
Molecular Weight : |
141.5kDa |
Activity : |
Measuredby the ability of the immobilized protein to enhance the adhesion of H4 humanneuroglioma cells. When 5 x 104 cells per well are added to recombinanthuman NCAN coated plates, cell adhesion is enhanced in a dose dependentmanner. The ED50 for this effect is typically 50-200 ng/mL. |
Endotoxin Level : |
<1.0 EUper 1 μg of the protein by the LAL method. |
SDS-PAGE : |
140-290kDa, reducing conditions. |
Purity : |
>90%,by SDS-PAGE under reducing conditions and visualized by silver stain. |
Reconstitution : |
100μg/mL in PBS |
Storage : |
Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months fromdate of receipt, -20 to -70°C as supplied. 1 month, 2 to 8°C under sterileconditions after reconstitution. 3 months, -20 to -70°C under sterileconditions after reconstitution. |