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| Cat. No.: | NCAN-5691H |
| Product Overview: | Recombinant Human NCANprotein was expressed in CHO with a C-terminal 6-His tag. Glu23-Cys1321. |
| Description: | NCAN, also known asCSPG3 and 1D1, is a secreted chondroitin sulfate proteoglycan that isprimarily expressed in the central nervous system. Human NCAN contains oneIg-like V-type domain, two Link domains, two EGF-like domains, one C-typelectin like domain, and one Sushi domain. It is an approximately 300 kDamolecule of which 66 kDa is chondroitin sulfate and 60 kDa is Nand O-linked glycosylation.Mature human NCAN shares 66% aa sequence identity with mature mouse and ratNCAN. |
| Source: | Chinese HamsterOvary cell line, CHO-derived. |
| Form: | Lyophilized from a0.2 μm filtered solution in PBS. |
| N-terminalSequence: | Glu23 |
| Molecular Weight: | 141.5kDa |
| Activity: | Measuredby the ability of the immobilized protein to enhance the adhesion of H4 humanneuroglioma cells. When 5 x 104 cells per well are added to recombinanthuman NCAN coated plates, cell adhesion is enhanced in a dose dependentmanner. The ED50 for this effect is typically 50-200 ng/mL. |
| Endotoxin Level: | <1.0 EUper 1 μg of the protein by the LAL method. |
| SDS-PAGE: | 140-290kDa, reducing conditions. |
| Purity: | >90%,by SDS-PAGE under reducing conditions and visualized by silver stain. |
| Reconstitution: | 100μg/mL in PBS |
| Storage: | Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months fromdate of receipt, -20 to -70°C as supplied. 1 month, 2 to 8°C under sterileconditions after reconstitution. 3 months, -20 to -70°C under sterileconditions after reconstitution. |
| Gene Name: | NCAN neurocan [ Homo sapiens] |
| Official Symbol: | NCAN |
| Synonyms: | NCAN; neurocan; CSPG3;FLJ44681; neurocan core protein; neurocan proteoglycan; chondroitin sulfateproteoglycan 3 (neurocan); NEUR; Chondroitin sulfate proteoglycan 3 |
| Gene ID: | 1463 |
| mRNA Refseq: | NM_004386 |
| Protein Refseq: | NP_004377 |
| MIM: | 600826 |
| UniProt ID: | O14594 |
| Chromosome Location: | 19p12 |
| Pathway: | Axon guidance; Developmental Biology; L1CAMinteractions; NCAM signaling for neurite out-growth; NCAM1 interactions;Syndecan-3-mediated signaling events |
| Function: | binding; calcium ion binding; hyaluronicacid binding; sugar binding |
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