Active Recombinant Human Neurocan, His-tagged

• Cat.No.: NCAN-5691H
• Product Overview: Recombinant Human NCANprotein was expressed in CHO with a C-terminal 6-His tag. Glu23-Cys1321.

Specification

• Cat. No.: NCAN-5691H
• Description: NCAN, also known asCSPG3 and 1D1, is a secreted chondroitin sulfate proteoglycan that isprimarily expressed in the central nervous system. Human NCAN contains oneIg-like V-type domain, two Link domains, two EGF-like domains, one C-typelectin like domain, and one Sushi domain. It is an approximately 300 kDamolecule of which 66 kDa is chondroitin sulfate and 60 kDa is Nand O-linked glycosylation.Mature human NCAN shares 66% aa sequence identity with mature mouse and ratNCAN.
• Source: Chinese HamsterOvary cell line, CHO-derived.
• Form: Lyophilized from a0.2 μm filtered solution in PBS.
• N-terminalSequence: Glu23
• Molecular Weight: 141.5kDa
• Activity: Measuredby the ability of the immobilized protein to enhance the adhesion of H4 humanneuroglioma cells. When 5 x 104 cells per well are added to recombinanthuman NCAN coated plates, cell adhesion is enhanced in a dose dependentmanner. The ED50 for this effect is typically 50-200 ng/mL.
• Endotoxin Level: <1.0 EUper 1 μg of the protein by the LAL method.
• SDS-PAGE: 140-290kDa, reducing conditions.
• Purity: >90%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: 100μg/mL in PBS
• Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months fromdate of receipt, -20 to -70°C as supplied. 1 month, 2 to 8°C under sterileconditions after reconstitution. 3 months, -20 to -70°C under sterileconditions after reconstitution.

Gene Information

• Gene Name: NCAN neurocan [ Homo sapiens]
• Official Symbol: NCAN
• Synonyms: NCAN; neurocan; CSPG3;FLJ44681; neurocan core protein; neurocan proteoglycan; chondroitin sulfateproteoglycan 3 (neurocan); NEUR; Chondroitin sulfate proteoglycan 3
• Gene ID: 1463
• mRNA Refseq: NM_004386
• Protein Refseq: NP_004377
• MIM: 600826
• UniProt ID: O14594
• Chromosome Location: 19p12
• Pathway: Axon guidance; Developmental Biology; L1CAMinteractions; NCAM signaling for neurite out-growth; NCAM1 interactions;Syndecan-3-mediated signaling events
• Function: binding; calcium ion binding; hyaluronicacid binding; sugar binding