| Cat. No.: | AF251705-534M |
| Product Overview: | Recombinant Mouse AF251705protein was expressed in Chinese Hamster Ovary cell line with the Fc part of MouseLMIR2 Cys22-Ser193 (N-terminus) and Mouse IgG2A Glu98-Lys330 (C-terminus). |
| Description: | AF251705, also known as CMRF35A, CD300c, CLM4, DIgR1, and MAIRII,is a 35 kDa type I transmembrane glycoprotein that belongs to theimmunoregulatory signaling (IRS) family of immunoglobulinlike molecules.Mature mouse AF251705 consists of a 165amino acid (aa) extracellular domain (ECD) with one Ig-like domain, a 21 aatransmembrane segment, and a 20 aa cytoplasmic tail. An alternate splice formlacks two amino acids C-terminal to the Ig-like domain. Within the ECD, mouseAF251705 shares 45% aa sequence identitywith human AF251705. |
| Source: | Chinese HamsterOvary cell line, CHO-derived. |
| Form: | Lyophilized from a0.2 μm filtered solution in PBS. |
| N-terminalSequence: | Cys22 |
| Molecular Weight: | 46 kDa(monomer) |
| Activity: | Measuredby its ability to inhibit anti-CD3-induced proliferation of stimulated human Tcells. Human T lymphocytes cultured for 72 hours with PHA were incubated foran additional 3 days in 96 well plates coated with 500 ng/mL antiCD3 andserially diluted rmLMIR-2/Fc. The ED50 for this effect istypically 0.6-3 μg/mL. |
| Endotoxin Level: | < 1.0EU per 1 μg of the protein by the LAL method. |
| SDS-PAGE: | 55-65 kDa,reducing conditions. |
| Purity: | > 95%,by SDSPAGE under reducing conditions and visualized by silver stain. |
| Reconstitution: | 100μg/mL in PBS. |
| Storage: | Use a manualdefrost freezer and avoid repeated freeze-thaw cycles. 12 months from date ofreceipt, -20 to -70°C as supplied. 1 month, 2 to 8°C under sterile conditionsafter reconstitution. 3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Gene Name: | AF251705 cDNA sequenceAF251705 [ Mus musculus ] |
| Official Symbol: | AF251705 |
| Synonyms: | AF251705; cDNAsequence AF251705; CLM4; DIgR1; Igsf7; Lmir2; Cd300c; Cd300d; MAIR-II; CMRF35-likemolecule; CLM; MAIR-2; Cd300C antigen; Cd300D antigen; leukocyte mono-Ig-likereceptor 2; immunoglobulin superfamily member 7; immunoglobulin superfamily,member 7; dendritic cell-derived Ig-like receptor 1; dendritic cell-derivedimmunoglobulin receptor 1; myeloid-associated immunoglobulin-like receptor 2;myeloid-associated immunoglobulin-like receptor II |
| Gene ID: | 140497 |
| mRNA Refseq: | NM_134158 |
| Protein Refseq: | NP_598919 |
| Chromosome Location: | 11 E2; 11 78.0 cM |
| Function: | protein binding;receptor activity |
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