| Cat. No. : | AF251705-534M |
| Product Overview : | Recombinant Mouse AF251705protein was expressed in Chinese Hamster Ovary cell line with the Fc part of MouseLMIR2 Cys22-Ser193 (N-terminus) and Mouse IgG2A Glu98-Lys330 (C-terminus). |
| Description : | AF251705, also known as CMRF35A, CD300c, CLM4, DIgR1, and MAIRII,is a 35 kDa type I transmembrane glycoprotein that belongs to theimmunoregulatory signaling (IRS) family of immunoglobulinlike molecules.Mature mouse AF251705 consists of a 165amino acid (aa) extracellular domain (ECD) with one Ig-like domain, a 21 aatransmembrane segment, and a 20 aa cytoplasmic tail. An alternate splice formlacks two amino acids C-terminal to the Ig-like domain. Within the ECD, mouseAF251705 shares 45% aa sequence identitywith human AF251705. |
| Source : | Chinese HamsterOvary cell line, CHO-derived. |
| Form : | Lyophilized from a0.2 μm filtered solution in PBS. |
| N-terminalSequence : | Cys22 |
| Molecular Weight : | 46 kDa(monomer) |
| Activity : | Measuredby its ability to inhibit anti-CD3-induced proliferation of stimulated human Tcells. Human T lymphocytes cultured for 72 hours with PHA were incubated foran additional 3 days in 96 well plates coated with 500 ng/mL antiCD3 andserially diluted rmLMIR-2/Fc. The ED50 for this effect istypically 0.6-3 μg/mL. |
| Endotoxin Level : | < 1.0EU per 1 μg of the protein by the LAL method. |
| SDS-PAGE : | 55-65 kDa,reducing conditions. |
| Purity : | > 95%,by SDSPAGE under reducing conditions and visualized by silver stain. |
| Reconstitution : | 100μg/mL in PBS. |
| Storage : | Use a manualdefrost freezer and avoid repeated freeze-thaw cycles. 12 months from date ofreceipt, -20 to -70°C as supplied. 1 month, 2 to 8°C under sterile conditionsafter reconstitution. 3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Gene Name : | AF251705 cDNA sequenceAF251705 [ Mus musculus ] |
| Official Symbol : | AF251705 |
| Synonyms : | AF251705; cDNAsequence AF251705; CLM4; DIgR1; Igsf7; Lmir2; Cd300c; Cd300d; MAIR-II; CMRF35-likemolecule; CLM; MAIR-2; Cd300C antigen; Cd300D antigen; leukocyte mono-Ig-likereceptor 2; immunoglobulin superfamily member 7; immunoglobulin superfamily,member 7; dendritic cell-derived Ig-like receptor 1; dendritic cell-derivedimmunoglobulin receptor 1; myeloid-associated immunoglobulin-like receptor 2;myeloid-associated immunoglobulin-like receptor II |
| Gene ID : | 140497 |
| mRNA Refseq : | NM_134158 |
| Protein Refseq : | NP_598919 |
| Chromosome Location : | 11 E2; 11 78.0 cM |
| Function : | protein binding;receptor activity |
Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.
Download Datasheet
See All AF251705 Products
Download Datasheet