Active Recombinant Mouse cDNA sequence AF251705, Fc-tagged

• Cat.No.: AF251705-534M
• Product Overview: Recombinant Mouse AF251705protein was expressed in Chinese Hamster Ovary cell line with the Fc part of MouseLMIR2 Cys22-Ser193 (N-terminus) and Mouse IgG2A Glu98-Lys330 (C-terminus).

Specification

• Species: Mouse
• Source: CHO
• Tag: Fc
• Protein Length: 22-193;98-330 a.a.
• Description: AF251705, also known as CMRF35A, CD300c, CLM4, DIgR1, and MAIRII,is a 35 kDa type I transmembrane glycoprotein that belongs to theimmunoregulatory signaling (IRS) family of immunoglobulinlike molecules.Mature mouse AF251705 consists of a 165amino acid (aa) extracellular domain (ECD) with one Ig-like domain, a 21 aatransmembrane segment, and a 20 aa cytoplasmic tail. An alternate splice formlacks two amino acids C-terminal to the Ig-like domain. Within the ECD, mouseAF251705 shares 45% aa sequence identitywith human AF251705.
• Form: Lyophilized from a0.2 μm filtered solution in PBS.
• N-terminalSequence: Cys22
• Molecular Weight: 46 kDa(monomer)
• Activity: Measuredby its ability to inhibit anti-CD3-induced proliferation of stimulated human Tcells. Human T lymphocytes cultured for 72 hours with PHA were incubated foran additional 3 days in 96 well plates coated with 500 ng/mL antiCD3 andserially diluted rmLMIR-2/Fc. The ED50 for this effect istypically 0.6-3 μg/mL.
• Endotoxin Level: < 1.0EU per 1 μg of the protein by the LAL method.
• SDS-PAGE: 55-65 kDa,reducing conditions.
• Purity: > 95%,by SDSPAGE under reducing conditions and visualized by silver stain.
• Reconstitution: 100μg/mL in PBS.
• Storage: Use a manualdefrost freezer and avoid repeated freeze-thaw cycles. 12 months from date ofreceipt, -20 to -70°C as supplied. 1 month, 2 to 8°C under sterile conditionsafter reconstitution. 3 months, -20 to -70°C under sterile conditions after reconstitution.

Gene Information

• Gene Name: AF251705 cDNA sequenceAF251705 [ Mus musculus ]
• Official Symbol: AF251705
• Synonyms: AF251705; cDNAsequence AF251705; CLM4; DIgR1; Igsf7; Lmir2; Cd300c; Cd300d; MAIR-II; CMRF35-likemolecule; CLM; MAIR-2; Cd300C antigen; Cd300D antigen; leukocyte mono-Ig-likereceptor 2; immunoglobulin superfamily member 7; immunoglobulin superfamily,member 7; dendritic cell-derived Ig-like receptor 1; dendritic cell-derivedimmunoglobulin receptor 1; myeloid-associated immunoglobulin-like receptor 2;myeloid-associated immunoglobulin-like receptor II
• Gene ID: 140497
• mRNA Refseq: NM_134158
• Protein Refseq: NP_598919
• Chromosome Location: 11 E2; 11 78.0 cM
• Function: protein binding;receptor activity