Active Recombinant Mouse cDNA sequence AF251705, Fc-tagged

• Cat.No.: AF251705-534M
• Product Overview: Recombinant Mouse AF251705protein was expressed in Chinese Hamster Ovary cell line with the Fc part of MouseLMIR2 Cys22-Ser193 (N-terminus) and Mouse IgG2A Glu98-Lys330 (C-terminus).

Specification

• Cat. No.: AF251705-534M
• Description: AF251705, also known as CMRF35A, CD300c, CLM4, DIgR1, and MAIRII,is a 35 kDa type I transmembrane glycoprotein that belongs to theimmunoregulatory signaling (IRS) family of immunoglobulinlike molecules.Mature mouse AF251705 consists of a 165amino acid (aa) extracellular domain (ECD) with one Ig-like domain, a 21 aatransmembrane segment, and a 20 aa cytoplasmic tail. An alternate splice formlacks two amino acids C-terminal to the Ig-like domain. Within the ECD, mouseAF251705 shares 45% aa sequence identitywith human AF251705.
• Source: Chinese HamsterOvary cell line, CHO-derived.
• Form: Lyophilized from a0.2 μm filtered solution in PBS.
• N-terminalSequence: Cys22
• Molecular Weight: 46 kDa(monomer)
• Activity: Measuredby its ability to inhibit anti-CD3-induced proliferation of stimulated human Tcells. Human T lymphocytes cultured for 72 hours with PHA were incubated foran additional 3 days in 96 well plates coated with 500 ng/mL antiCD3 andserially diluted rmLMIR-2/Fc. The ED50 for this effect istypically 0.6-3 μg/mL.
• Endotoxin Level: < 1.0EU per 1 μg of the protein by the LAL method.
• SDS-PAGE: 55-65 kDa,reducing conditions.
• Purity: > 95%,by SDSPAGE under reducing conditions and visualized by silver stain.
• Reconstitution: 100μg/mL in PBS.
• Storage: Use a manualdefrost freezer and avoid repeated freeze-thaw cycles. 12 months from date ofreceipt, -20 to -70°C as supplied. 1 month, 2 to 8°C under sterile conditionsafter reconstitution. 3 months, -20 to -70°C under sterile conditions after reconstitution.

Gene Information

• Gene Name: AF251705 cDNA sequenceAF251705 [ Mus musculus ]
• Official Symbol: AF251705
• Synonyms: AF251705; cDNAsequence AF251705; CLM4; DIgR1; Igsf7; Lmir2; Cd300c; Cd300d; MAIR-II; CMRF35-likemolecule; CLM; MAIR-2; Cd300C antigen; Cd300D antigen; leukocyte mono-Ig-likereceptor 2; immunoglobulin superfamily member 7; immunoglobulin superfamily,member 7; dendritic cell-derived Ig-like receptor 1; dendritic cell-derivedimmunoglobulin receptor 1; myeloid-associated immunoglobulin-like receptor 2;myeloid-associated immunoglobulin-like receptor II
• Gene ID: 140497
• mRNA Refseq: NM_134158
• Protein Refseq: NP_598919
• Chromosome Location: 11 E2; 11 78.0 cM
• Function: protein binding;receptor activity

Reviews

12/22/2021

Elucidating protein-protein interactions in protein folding and misfolding diseases.

12/27/2018

Deciphering protein-protein interactions in metabolic disorders for therapeutic interventions.

04/21/2017

Exploring protein-protein interactions in cell cycle regulation for understanding cell division.

Q&As

Are there any genetic variations or mutations in the gene associated with AF251705 protein that impact its expression or function, and what are the implications for cellular processes or disease susceptibility?

Genetic variations or mutations in the gene associated with AF251705 protein may impact its expression or function, potentially affecting cellular processes or disease susceptibility.

How does AF251705 protein contribute to disease development or pathophysiological conditions, and what are the underlying molecular mechanisms?

AF251705 protein may contribute to disease development or pathophysiological conditions, and the underlying molecular mechanisms are currently being investigated.

What is the subcellular localization of AF251705 protein, and how can it be experimentally determined?

The subcellular localization of AF251705 protein can be determined experimentally using techniques such as immunofluorescence microscopy or subcellular fractionation.

What experimental techniques or assays have been used to study the functional significance of AF251705 protein?

Various experimental techniques or assays have been used to study the functional significance of AF251705 protein, including functional genomics approaches, protein-protein interaction studies, or cellular assays.

How does dysregulation or dysfunction of AF251705 protein affect cellular homeostasis, molecular signaling networks, or disease progression?

Dysregulation or dysfunction of AF251705 protein can disrupt cellular homeostasis, perturb molecular signaling networks, and contribute to disease progression.

Does AF251705 protein interact with specific molecules or participate in protein complexes, and what are the functional implications of these interactions?

AF251705 protein may interact with specific molecules or participate in protein complexes, influencing various cellular functions and processes.

What is the role of AF251705 protein in cellular processes, and how can its function be investigated experimentally?

The role of AF251705 protein in cellular processes can be investigated experimentally using functional assays or loss-of-function/gain-of-function studies.

Are there any known post-translational modifications or regulatory mechanisms that modulate the activity or stability of AF251705 protein?

Post-translational modifications and regulatory mechanisms may modulate the activity or stability of AF251705 protein.

How is the expression of AF251705 protein regulated, and are there any factors or signaling pathways involved in its expression control?

The expression of AF251705 protein is regulated, and there may be specific factors or signaling pathways involved in its expression control.

Can AF251705 protein be targeted or manipulated to modulate cellular processes or serve as a potential therapeutic target?

AF251705 protein holds the potential to be targeted or manipulated to modulate cellular processes or serve as a potential therapeutic target, warranting further investigation for its therapeutic applications.