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| Cat.No.: | CAB11477MH |
| Product Overview: | Mouse monoclonal antibody to human acrosomal vesicle protein 1. |
| Antigen Description: | Acrosomal protein SP-10, also known as Acrosomal vesicle protein 1 and ACRV1, is a testis-specific, differentiation antigen, that arises within the acrosomal vesicle during spermatogenesis, and is associated with the acrosomal membranes and matrix of mature sperm. Regulation of cell type-specific gene transcription is central to cellular differentiation and development. During spermatogenesis, a number of testis-specific genes are expressed in a precise spatiotemporal order. The longest transcript of ACRV1 / SP-10 is the most abundant, comprising 53-72% of the total acrosomal vesicle protein 1 messages; the second largest transcript comprises 15-32%; the third and the fourth largest transcripts account for 3.4-8.3% and 8.7-12.5%, respectively; and the remaining transcripts combined account for< 1% of the total acrosomal vesicle protein 1 message. acrv1 sp-10 is a testis-specific acrosomal protein that has been detected in several species including humans. it may be involved in sperm-zona binding or penetration, and it is a potential contraceptive vaccine immunogen for humans. acrv1 sp-10 may be involved in sperm-zona binding or penetration. it is also a intra-acrosomal protein that is considered to be a vaccine candidate for> |
| Specificity: | Human ACRV1. No cross-reactivity with human cell lysate ( 293 cell line ) in ELISA. |
| Immunogen: | Recombinant Human ACRV1 Protein |
| Host: | Mouse |
| Isotype: | Mouse IgG1 |
| Species: | Human |
| Clone: | 4E3G11 |
| Purification: | Protein A affinity chromatography |
| Applications: | Western Blot; ELISA |
| Dilution: | Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect Human ACRV1 in WB. Using a DAB detection system, the detection limit for Human ACRV1 is approximately 20 ng/lane under non-reducing conditions and reducing conditions.Direct ELISA: This antibody can be used at 0.5 - 1 μg with the appropriate secondary reagents to detect Human ACRV1. The detection limit for Human ACRV1 is approximately 0.0195 ng/well. |
| Preparation: | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, human cell-derived, recombinant Human ACRV1. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Format: | 0.2 μm filtered solution in PBS with 5% trehalose |
| Storage: | This antibody can be stored at 2-8 °C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 °C to -70 °C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. |
| Gene Name: | ACRV1 acrosomal vesicle protein 1 [ Homo sapiens ] |
| Official Symbol: | ACRV1 |
| Synonyms: | ACRV1; acrosomal vesicle protein 1; acrosomal protein SP-10; D11S4365; SP 10; SPACA2; sperm protein 10; SP-10; |
| Gene ID: | 56 |
| mRNA Refseq: | NM_001612 |
| Protein Refseq: | NP_001603 |
| MIM: | 102525 |
| UniProt ID: | P26436 |
| Chromosome Location: | 11p12-q13 |
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