| Species : |
Human |
| Source : |
Mouse |
| Tag : |
Non |
| Antigen Description : |
Acrosomal protein SP-10, also known as Acrosomal vesicle protein 1 and ACRV1, is a testis-specific, differentiation antigen, that arises within the acrosomal vesicle during spermatogenesis, and is associated with the acrosomal membranes and matrix of mature sperm. Regulation of cell type-specific gene transcription is central to cellular differentiation and development. During spermatogenesis, a number of testis-specific genes are expressed in a precise spatiotemporal order. The longest transcript of ACRV1 / SP-10 is the most abundant, comprising 53-72% of the total acrosomal vesicle protein 1 messages; the second largest transcript comprises 15-32%; the third and the fourth largest transcripts account for 3.4-8.3% and 8.7-12.5%, respectively; and the remaining transcripts combined account for< 1%="" of="" the="" total="" acrosomal="" vesicle="" protein="" 1="" message.="" acrv1="" sp-10="" is="" a="" testis-specific="" acrosomal="" protein="" that="" has="" been="" detected="" in="" several="" species="" including="" humans.="" it="" may="" be="" involved="" in="" sperm-zona="" binding="" or="" penetration,="" and="" it="" is="" a="" potential="" contraceptive="" vaccine="" immunogen="" for="" humans.="" acrv1="" sp-10="" may="" be="" involved="" in="" sperm-zona="" binding="" or="" penetration.="" it="" is="" also="" a="" intra-acrosomal="" protein="" that="" is="" considered="" to="" be="" a="" vaccine="" candidate="" for=""> |
| Specificity : |
Human ACRV1. No cross-reactivity with human cell lysate ( 293 cell line ) in ELISA. |
| Immunogen : |
Recombinant Human ACRV1 Protein |
| Isotype : |
Mouse IgG1 |
| Clone : |
4E3G11 |
| Purification : |
Protein A affinity chromatography |
| Applications : |
Western Blot; ELISA |
| Dilution : |
Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect Human ACRV1 in WB. Using a DAB detection system, the detection limit for Human ACRV1 is approximately 20 ng/lane under non-reducing conditions and reducing conditions.Direct ELISA: This antibody can be used at 0.5 - 1 μg with the appropriate secondary reagents to detect Human ACRV1. The detection limit for Human ACRV1 is approximately 0.0195 ng/well. |
| Preparation : |
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, human cell-derived, recombinant Human ACRV1. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Format : |
0.2 μm filtered solution in PBS with 5% trehalose |
| Storage : |
This antibody can be stored at 2-8 °C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 °C to -70 °C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. |
