Active Recombinant Human TBK1, GST-tagged

• Cat.No.: TBK1-1493H
• Product Overview: Recombinant full-length human TBK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Protein Length: Full length
• Description: TBK1, also known as NAK or NFkB-activating kinase, is an upstream protein kinase that can phosphorylate and activate the IkB kinases. Activation of IkB kinases allows the phosphorylation of IkB protein which is then degraded via the ubiquitination pathway. This mechanism allows the activation of the NFkB transcriptional complex. TBK1 is a specific upstream regulator of IkB kinases and can also interact and the IkB protein TANK. TBK1 is a component of the virus-activated kinase that phosphorylate IRF3 and IRF7 allowing their dimerization and translocation to the nucleus, where they induce transcription of interferon.
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Bio-activity: 282 nmol/min/mg
• Molecular Mass: ~105 kDa
• Purity: >80%
• Applications: Kinase Assay, Western Blot
• Storage: Store at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. Avoid freeze/thaw cycles.
• Concentration: 0.1μg/μl

Gene Information

• Gene Name: TBK1 TANK-binding kinase 1 [ Homo sapiens ]
• Official Symbol: TBK1
• Synonyms: TBK1; TANK-binding kinase 1; serine/threonine-protein kinase TBK1; NAK; NF-kB-activating kinase; NF-kappa-B-activating kinase; T2K; FLJ11330
• Gene ID: 29110
• mRNA Refseq: NM_013254
• Protein Refseq: NP_037386
• MIM: 604834
• UniProt ID: Q9UHD2
• Chromosome Location: 12q14.2
• Pathway: Activated TLR4 signalling, organism-specific biosystem; Activation of IRF3/IRF7 mediated by TBK1/IKK epsilon, organism-specific biosystem; Cytosolic DNA-sensing pathway, organism-specific biosystem; Cytosolic DNA-sensing pathway, conserved biosystem; Cytosolic sensors of pathogen-associated DNA, organism-specific biosystem; DAI mediated induction of type I IFNs, organism-specific biosystem; Hepatitis C, organism-specific biosystem
• Function: ATP binding; nucleic acid binding; nucleotide binding; phosphoprotein binding; protein binding; protein kinase activity; protein serine/threonine kinase activity

Citation

Quantitation of the Dynamic Profiles of the Innate Immune Response Using Multiplex Selected Reaction Monitoring-Mass Spectrometry

Journal: Molecular & Cellular Proteomics : MCP    PubMed ID: 23418394    Data: 2014/6/1

Authors: Yingxin Zhao, Bing Tian, Allan R. Brasier

Article Snippet:The recombinant proteins full-length human GST-tagged IKKγ and GST-tagged IκBα were made by our laboratory.The recombinant proteins full-length human GST-tagged IKKγ and GST-tagged IκBα were made by our laboratory.. GST-tagged human NFκB2 (1–454 amino acid), N-terminal proprietary tagged human IKKα, His-tagged human MAVS, GST-tagged human TBK1, and recombinant human Rela (1–537 amino acid) were purchased from Creative BioMart (Shirley, NY).. GST-tagged full-length human IRF3 was purchased from Abnova (Walnut, CA), and C-terminal MYC/DDK-tagged human RIG-I protein was purchased from Origene (Rockville, MD).GST-tagged full-length human IRF3 was purchased from Abnova (Walnut, CA), and C-terminal MYC/DDK-tagged human RIG-I protein was purchased from Origene (Rockville, MD).