Recombinant E.Coli Heat Shock Protein

• Cat.No.: grpE-4706E
• Product Overview: GrpE Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 197 amino acids and having a molecular mass of 21.8 kDa. The GrpE is purified by proprietary chromatographic techniques.

Specification

• Cat. No.: grpE-4706E
• Description: GrpE, co-chaperone of E.coli, participates actively in the response to hyperosomotic and heat shock by preventing the aggregation of stress-denatured proteins in association with DnaK. This protein is the nucleotide exchange factor for DnaK and may function as a thermosensor. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding.
• Source: E. Coli
• Host: Escherichia Coli
• Form: The HSP24 protein solution contains 20mM Tris-HCl pH-8 and 0.1M NaCl.
• Purity: Greater than 90.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Physical Appearance: Sterile filtered colorless solution.
• Amino acid sequence: MSSKEQKTPE GQAPEEIIMD QHEEIEAVEP EASAEQVDPR DEKIANLEAQ LAEAQTRERD GILRVKAEME NLRRRTELDI EKAHKFALEK FINELLPVID SLDRALEVAD KANPDMSAMV EGIELTLKSM LDVVRKFGVE VIAETNVPLD PNVHQAIAMV ESDDVAPGNV LGIMQKGYTL NGRTIRAAMV TVAKAKA.
• Storage: GrpE although stable 4°C for 4 weeks, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: grpE heat shock protein [ Escherichia coli str. K-12 substr. MG1655 ]
• Official Symbol: grpE
• Synonyms: grpE; heat shock protein; HSP24; HSPB25.3; HSP-70 Cofactor; Protein grpE; Heat shock protein B25.3; b2614; JW2594; ECK2610
• Gene ID: 947097
• Protein Refseq: NP_417104
• UniProt ID: P09372
• Function: protein folding