Mouse anti-His Monoclonal Antibody

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Mouse anti-His Monoclonal Antibody

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Cat. No. : CAB089
Antigen Information : A polyhistidine-tag is an amino acid motif in proteins that consists of at least six histidine (His) residues, often at the N- or C-terminus of the protein. It is also known as hexa histidine-tag, 6xHis-tag, and by the trademarked name His-tag (registered by EMD Biosciences). The tag was invented by Roche and its vectors are distributed by Qiagen. A Variety of purification kits for histidine-tagged proteins are available from Qiagen, Sigma, Thermo Scientific, GE Healthcare and others.The use of the tag for academic users is unrestricted; however, commercial users must pay royalties to Roche. Suitable tag sequences are available for free commercial use; for example, MK(HQ)6 may be used for enhanced expression in E. coli and tag removal. The total number of histidine residues may vary in the tag. The his-tag may also be followed by a suitable amino acid sequence that facilitates a removal of the polyhistidine-tag using endopeptidases. This extra sequence is not necessary if exopeptidases are used to remove N-terminal His-tags (e.g., Qiagen TAGZyme). Furthermore, exopeptidase cleavage may solve the unspecific cleavage observed when using endoprotease-based tag removal. Polyhistidine-tags are often used for affinity purification of genetically modified proteins.
Epitope : This antibody can recognize protein with His-tag in C-teminal.
application : E(1:5000); WB(1:1000)
Storage : One year at 4℃
<!--<img border="0" src="productimages/CUQ05 Ubc12 His.jpg" width="250" height="221" style="border: 2px solid #3E693F" alt="Anti-His,Mouse anti-His Monoclonal Antibody" /><a title="Protein Data Bank" style="color: #0F4B8C; text-decoration: none; text-underline: none; text-line-through: none" href="http://en.wikipedia.org/wiki/Protein_Data_Bank">PDB</a>rendering based on 1y8x.Available structures:<a title="http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1y8x" style="color: #0F4B8C; text-decoration: none; text-underline: none; text-line-through: none" href="http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1y8x">1y8x</a>,<a title="http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=2nvu" style="color: #0F4B8C; text-decoration: none; text-underline: none; text-line-through: none" href="http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=2nvu">2nvu</a>--> :

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