Recombinant Exendin-4 Exendin-4-01

Specification
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Specification
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Cat. No.:	Exendin-4-01
Product Overview:	Recombinant Exendin-4 produced inE.coliis a single non-glycosylated polypeptide chain containing 39 amino acids and having a molecular mass of approximately 4.1 kDa.
Description:	Exendin-4 is a novel 39-amino acid peptide isolated from the venom of the Gila monster Heloderma suspectum. It shares 53% sequence homology with GLP-17-36amide and interacts with the same membrane receptor. Exendin-4 enhances glucose-dependent insulin secretion, suppressesinappropriately elevated glucagon secretion, and slows gastricemptying in vivo. It also promotes ß-cell proliferationand neogenesis in vitro and in animal models. Recombinant Exendin-4 is E. coliexpression of a synthetic DNA squence encoding the 39 amino acid of Exendin-4.
Purity:	>96% by SDS-PAGE and HPLC analyses.
CAS Number:	141758-74-9
Molecular Formula:	C₁₈₄H₂₈₂N₅₀O₆₀S
Formulation:	Lyophilized from a 0.2mm filtered solution of 20mM PBS, pH 7.0, containing 4% mannitol.
Specific Activity:	Sterile Filtered White lyophilized (freeze-dried) powder.
Endotoxin:	Less than 10EU/mg of rExendin-4 as determined by LAL method.
Reconstitution:	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at<-20^oC. Further dilutions should be made in appropriate buffered solutions.
Storage:	This lyophilized preparation is stable at 2-8^oC, but should be kept at -20^oC for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8^oC. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20^oC. Avoid repeated freeze/thaw cycles.