Species : |
Human |
Source : |
Pichia |
Tag : |
Non |
Description : |
At least 23 different variants of Interferon-alpha are known. The individual proteins have molecular masses between 19,000-26,000 Da and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-alpha subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-alpha subtypes differ in their sequences at only one or two positions. Naturally occurring variants also include proteins truncated by 10 amino acids at the carboxyl-terminal end. |
Form : |
Lyophilized from a (1 mg/ml) solution in containing 5.55 mg sodium phosphate dibasic, 5.55 mg sodium phosphate monobasic buffer, 296 mg sucrose and 0.37 mg Tween 80. |
Bio-activity : |
Human HSA-IFN-α 2b is fully biologically active when compared to standard. The specific activity as determined in a viral resistance assay using bovine kidney MDBK cells was found to be 5×10⁷ IU/mg. |
Molecular Mass : |
85,700 Da |
Endotoxin : |
Less than 0.1 ng/μg (1 EU/μg) of human HSA-IFN-α 2b |
Purity : |
Greater than 98.0% as determined by:(a) Analysis by RP-HPLC(b) Anion-exchange FPLC(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel |
Storage : |
Lyophilized HSA-IFN-a2b although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution HSA-IFN-α 2b should be stored at 4°C between 2-7 days and for future use below -18°C. For long-term storage it is recommended to ass a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles. |
Reconstitution : |
It is recommended to reconstitute the lyophilized HSA-IFN a2b in sterile 18 MΩ-cm H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. |