Recombinant Human ERN1, Gly & Pro tagged

• Cat.No.: ERN1-466H
• Product Overview: Recombinant Human ERN1 (O75460-1) (Pro465-Leu977), fused with two additional amino acids (Gly & Pro) at the N-terminus, was produced in Baculovirus-Insect cells.

Specification

• Source: Baculovirus-Insect cells
• Species: Human
• Tag: Gly & Pro
• Predicted N Terminal: Gly
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.4.Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization.
• Molecular Mass: The secreted recombinant human ERN1 consists of 515 amino acids and predicts a molecular mass of 58.3 KDa. The apparent molecular mass of the protein is approximately 65 KDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 eu per μg of the protein as determined by the LAL method.
• Purity: >80 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70oC.
• Storage: Store it under sterile conditions at -20oC~-70oC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: ERN1 endoplasmic reticulum to nucleus signaling 1 [ Homo sapiens ]
• Official Symbol: ERN1
• Gene ID: 2081
• mRNA Refseq: NM_001433
• Protein Refseq: NP_001424
• MIM: 604033
• UniProt ID: O75460
• Chromosome Location: 17q23