Active Recombinant Human ITGAVB3

• Cat.No.: ITGAVB3-104H
• Product Overview: Recombinant Human ITGAVB3 protein (Human Integrin αV subunit (Accession# NP_002201, Phe31–Val992), and Human Integrin β3 subunit (Accession# AAA52589, Gly27–Asp718)) was co-expressed in CHO Cells.

Specification

• Description: This gene encodes a protein that is a member of the integrin superfamily. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This protein undergoes post-translational cleavage to yield disulfide-linked heavy and light chains that combine with multiple integrin beta chains to form different integrins. This protein has been shown to heterodimerize with beta 1, beta 3, beta 5, beta 6, and beta 8; the heterodimer of alpha v and beta 3 is the Vitronectin receptor. This protein interacts with several extracellular matrix proteins to mediate cell adhesion and may play a role in cell migration. It is proposed that this protein may regulate angiogenesis and cancer progression. Alternative splicing results in multiple transcript variants that encode different protein isoforms.
• Source: CHO Cells
• Species: Human
• Predicted N Terminal: Phe31 & Gly27
• Form: Lyophilized from 85.4 μL of a 0.2 μm filtered solution in 50 mM Tris, 150 mM NaCl and 0.5 mM CaCl2, pH 7.4.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human Vitronectin at 1 μg/mL can bind Recombinant Human Integrin αVβ3 with an apparent KD < 10 nM.
• Molecular Mass: Predicted Molecular Mass: 110.5 kDa ( alpha V subunit), 80.8 kDa ( beta 3 subunit)SDS-PAGE: 105-150 kDa, reducing conditions
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 °C as supplied.1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.

Gene Information

• Gene Name: ITGAV integrin, alpha V [ Homo sapiens (human) ]
• Official Symbol: ITGAV
• Synonyms: ITGAV; CD51; MSK8; VNRA; VTNR; integrin, alpha V; integrin alpha-V; integrin alphaVbeta3; vitronectin receptor subunit alpha; antigen identified by monoclonal antibody L230; integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51)
• Gene ID: 3685
• mRNA Refseq: NM_002210
• Protein Refseq: NP_002201
• MIM: 193210
• UniProt ID: P06756
• Chromosome Location: 2q31-q32
• Pathway: Adaptive Immune System; Antigen processing-Cross presentation; Arrhythmogenic right ventricular cardiomyopathy (ARVC)
• Function: contributes_to insulin-like growth factor I binding; contributes_to opsonin binding; contributes_to protein kinase C binding