3T3 NIH Whole Cell Lysate
Cat.No. : | NIH 3T3-151M |
- Specification
- Gene Information
- Related Products
Species : | Mouse |
Form : | The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol. |
Concentration : | 2.0 mg/ml |
Cell line : | NIH/3T3 (embryo) |
Tissue Type : | Fibroblast from NIH Swiss mouse embryos |
Growth media : | DMEM & 10% fetal bovine serum |
Preparation method : | Mouse NIH/3T3 cell lysate was prepared by homogenization in modified RIPA buffer (50 mM Tris-HCl, pH 7.4, 1% Triton X-100, 0.2% sodium deoxycholate, 0.2% sodium dodecylsulfate (SDS), 1 mM sodium ethylenediaminetetraacetate, 1 mM phenylmethyl-sulfonyl fluoride, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin)). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% SDS, 0.01% bromophenol blue) containing 5% β-mercaptoethanol. |
Expiration Date : | One year from date of receipt |
Size : | 0.1 mg, 0.5 mg |
Storage Instruction : | This whole cell lysate is stable for three months at -20°C or at -70°C for up to one year. |
Applications : | This lysate is for use in Western blotting, 10 μg to 20 μg per lane is recommended for mini gel. |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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