3T3 NIH Whole Cell Lysate NIH 3T3-151M

Cat.No. :	NIH 3T3-151M
Species :	Mouse
Form :	The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.
Concentration :	2.0 mg/ml
Cell line :	NIH/3T3 (embryo)
Tissue Type :	Fibroblast from NIH Swiss mouse embryos
Growth media :	DMEM & 10% fetal bovine serum
Preparation method :	Mouse NIH/3T3 cell lysate was prepared by homogenization in modified RIPA buffer (50 mM Tris-HCl, pH 7.4, 1% Triton X-100, 0.2% sodium deoxycholate, 0.2% sodium dodecylsulfate (SDS), 1 mM sodium ethylenediaminetetraacetate, 1 mM phenylmethyl-sulfonyl fluoride, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin)). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% SDS, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.
Expiration Date :	One year from date of receipt
Size :	0.1 mg, 0.5 mg
Storage Instruction :	This whole cell lysate is stable for three months at -20°C or at -70°C for up to one year.
Applications :	This lysate is for use in Western blotting, 10 μg to 20 μg per lane is recommended for mini gel.

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