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Active Recombinant Human Lysozyme

Cat.No. : LYZ-64H
Product Overview : Recombinant Human Lysozyme is added in lysing bacterial cells to the cell extraction buffer at a range of 0.05-0.5 mg/ml (7,000-70,000 U/ml). Recombinant Human Lysozyme produced in Plant is a single, non-glycosilated polypeptide chain containing 130 amino acids and a molecular mass of 14 kDa.
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Cat. No. : LYZ-64H
Description : Lysozyme is an enzyme found in egg white, tears, and other secretions. Lysozyme is responsible for attacking & breaking down the polysaccharide walls of many kinds of bacteria and thus it provides some protection against infection. Bacteria build a tough skin of carbohydrate chains, interlocked by short peptide strands, that braces their delicate membrane against the cell"s high osmotic pressure. Lysozyme breaks these carbohydrate chains, destroying the structural integrity of the cell wall. The bacteria burst under their own internal pressure. Lysozyme (muramidase) hydrolyzes preferentially the β-1,4 glucosidic linkages between N-acetylmuramic acid and N-acetylglucosamine which occur in the mucopeptide cell wall. Lysozyme is a mediator in the anti-tumor function of macrophages which, it has been shown, secrete the enzyme. Cartilage lysozyme has a role in cartilage calcification. Presence of the Lysozyme in cerebrospinal fluid is indicative of tumor of the central nervous system, normally lysozyme activity is practically absent from urine, bile and spinal fluid.
Source : Rice Grain
Physical Appearance : Sterile Filtered white lyophilized powder.
Purity : Greater than 85% as determined by both: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Formulation : The Recombinant Lysozyme was lyophilized with no additives.
Activity : 158,800 Units/mg protein. 1 unit will produce a change of A450 0.001 per minute at pH-6.24 and 25℃, using a suspension of micrococcus lysodeikticus as substrate in a 2.6ml reaction volume.
Stability : Recombinant Lysozyme although stable at room temperature for 3 weeks, should be stored at 2-8°C.
Instructions for Use : Recombinant Human Lysozyme is added in lysing bacterial cells to the cell extraction buffer at a range of 0.05-0.5mg/ml (7,000-70,000 U/ml). The common extraction buffer for E. coli cells contains 0.2mg/ml of recombinant Lysozyme in 100mM Tris-HCl, 2mM EDTA, 0.05% Triton X-100, pH 8. The cell paste is suspended in extraction buffer and incubated for at least 15 min at room temperature. Alternatively, Triton X-100 at 1% and PMSF at 1mM can be added separately and the lysis solution is further incubated for at least 15 min at 37 °C. In other cases, freeze-thaw cycles, sonication, and mechanical cell disruptions are also used to complement the lysozyme lysis protocol.
Synonyms : Lysozyme C; EC 3.2.1.17; LYZ; LZM; 1;4-beta-N-acetylmuramidase C; Lysozyme; lysozyme; 1,4-beta-N-acetylmuramidase C; lysozyme (renal amyloidosis); lysozyme C; muramidase; globulin G; mucopeptide glucohydrolase; globulin G1; N,O-diacetylmuramidase; lysozyme g; L-7001; 1,4-N-acetylmuramidase; mucopeptide N-acetylmuramoylhydrolase; PR1-lysozyme; peptidoglycan N-acetylmuramoylhydrolase.
Synonyms : Lysozyme C; EC 3.2.1.17; LYZ; LZM; 1;4-beta-N-acetylmuramidase C; Lysozyme; lysozyme; 1,4-beta-N-acetylmuramidase C; lysozyme (renal amyloidosis); lysozyme C; muramidase; globulin G; mucopeptide glucohydrolase; globulin G1; N,O-diacetylmuramidase; lysozyme g; L-7001; 1,4-N-acetylmuramidase; mucopeptide N-acetylmuramoylhydrolase; PR1-lysozyme; peptidoglycan N-acetylmuramoylhydrolase.

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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Can LYZ be used to treat gastrointestinal infections? 05/25/2021

Some research suggests that LYZ may have potential in treating gastrointestinal infections, but more studies are needed.

Can LYZ be used to treat common colds? 03/03/2020

While LYZ has antibacterial properties, it is not typically used to treat viral infections like the common cold.

How is LYZ protein used in the food industry? 07/05/2019

LYZ is used in the food industry as a natural preservative to prevent the growth of bacteria in various food products.

What are the clinical applications of LYZ protein? 02/17/2019

LYZ protein has several clinical applications, including in the field of medicine and biotechnology.

Are there any side effects associated with LYZ therapy? 08/17/2018

LYZ therapy is generally considered safe, but there may be rare allergic reactions or side effects in some individuals.

Customer Reviews (3)

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Reviews
07/21/2022

    when employed in Western Blotting experiments, the LYZ protein consistently generates sharp and well-defined protein bands, enabling precise visualization and analysis of protein expression.

    01/13/2020

      I am extremely pleased with the quality of the LYZ protein as it perfectly fulfills my experimental requirements.

      03/26/2018

        This protein exhibits exceptional purity and reliability, ensuring accurate and reproducible results in my assays.

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