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Recombinant Human ERBB2, His-tagged

Cat.No. : ERBB2-211H
Product Overview : Recombinant Human HER2 /ErbB2 MS Standard Protein, C13 and N15-labeled (HER2 / ErbB2, Heavy Labeled) Thr 23 - Thr 652 (Accession # AAA75493) was produced in human 293 cells (HEK293) with fully chemically defined cell culture medium to obtain >99% incorpor
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Description : Human Epidermal growth factor Receptor 2 (HER2) is also called ERBB2, HER-2,HER-2 /neu, NEU, NGL,TKR1 and c-erb B2,and is a protein giving higher aggressiveness in breast cancers. It is a member of the ErbB protein family, more commonly known as the epidermal growth factor receptor family. HER2 is a cell membrane surface-bound receptor tyrosine kinase and is normally involved in the signal transduction pathways leading to cell growth and differentiation. HER2 is thought to be an orphan receptor, with none of the EGF family of ligands able to activate it. Approximately 30% of breast cancers have an amplification of the HER2 gene or overexpression of its protein product. Overexpression of this receptor in breast cancer is associated with increased disease recurrence and worse prognosis. HER2 appears to play roles in development, cancer,communication at the neuromuscular junction andregulation of cell growth and differentiation.
Source : HEK293
Species : Human
Tag : His
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
Molecular Mass : rh HER2 / ErbB2, Heavy Labeled is fused with polyhistidine tag at the C-terminus, and has a calculated MW of 72.4 kDa. The predicted N-terminus is Thr 23. DTT-reduced Protein migrates as 90-110 kDa in SDS-PAGE due to glycosylation.HER2 / ErbB2, Heavy Lab
Endotoxin : Less than 1.0 EU per μg of the rh HER2 / ErbB2, Heavy Labeled by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Storage : Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).
Reconstitution : See Certificate of Analysis for reconstitution instructions and specific concentrations.
Gene Name : ERBB2 v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) [ Homo sapiens ]
Official Symbol : ERBB2
Synonyms : ERBB2; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian); NGL, v erb b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog); receptor tyrosine-protein kinase erbB-2; CD340; HER 2; HER2; NEU; herstatin; p185erbB2; proto-oncogene Neu; c-erb B2/neu protein; proto-oncogene c-ErbB-2; metastatic lymph node gene 19 protein; tyrosine kinase-type cell surface receptor HER2; neuroblastoma/glioblastoma derived oncogene homolog; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog); NGL; TKR1; HER-2; MLN 19; HER-2/neu;
Gene ID : 2064
mRNA Refseq : NM_001005862
Protein Refseq : NP_001005862
MIM : 164870
UniProt ID : P04626
Chromosome Location : 17q11.2-q12
Pathway : Adherens junction, organism-specific biosystem; Adherens junction, conserved biosystem; Alpha6-Beta4 Integrin Signaling Pathway, organism-specific biosystem; Axon guidance, organism-specific biosystem; Bladder cancer, organism-specific biosystem; Bladder cancer, conserved biosystem; Calcium signaling pathway, organism-specific biosystem;
Function : ATP binding; ErbB-3 class receptor binding; Hsp90 protein binding; RNA polymerase I core binding; epidermal growth factor-activated receptor activity; glycoprotein binding; contributes_to growth factor binding; identical protein binding; nucleotide binding; protein C-terminus binding; protein binding; protein dimerization activity; protein heterodimerization activity; protein heterodimerization activity; protein heterodimerization activity; protein phosphatase binding; protein tyrosine kinase activity; protein tyrosine kinase activity; protein tyrosine kinase activity; receptor activity; receptor signaling protein tyrosine kinase activity; transmembrane receptor protein tyrosine kinase activity; transmembrane signaling receptor activity; ubiquitin protein ligase binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (8)

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Do you have a recommended protocol for immobilizing any avi-tagged recombinant proteins on a streptavidin coated plate? 02/01/2023

1.Prepare the streptavidin-coated plate by washing it with PBS (phosphate-buffered saline) or another suitable buffer to remove any impurities. 2.Dilute the Avi-tagged protein in the desired buffer at the desired concentration. It is important to use a buffer that is compatible with the protein and does not interfere with its activity or stability. 3.Add the diluted protein to the streptavidin-coated plate and incubate it for a suitable amount of time at room temperature or 4°C. The exact conditions will depend on the specific protein and the desired level of immobilization. 4.Wash the plate with a suitable buffer to remove any unbound protein. 5.Block the remaining binding sites on the plate with a suitable blocking agent such as BSA (bovine serum albumin) or casein. 6.Wash the plate again to remove any unbound blocking agent. 7.Use the immobilized protein for further experiments such as ELISA, Western blotting, or other assays.

What is the primary role of ERBB2 in cell signaling? 08/12/2022

ERBB2 is a receptor tyrosine kinase involved in the regulation of cell growth and differentiation.

What therapeutic agents target ERBB2 in cancer treatment? 10/03/2020

Therapeutic agents targeting ERBB2 include trastuzumab and lapatinib.

In which types of cancer is ERBB2 commonly overexpressed? 05/12/2020

ERBB2 is commonly overexpressed in breast, ovarian, and stomach cancers.

How does ERBB2 overexpression affect patient prognosis in cancer? 05/04/2019

ERBB2 overexpression is often associated with aggressive disease and poor prognosis.

How can ERBB2 levels be assessed in a clinical setting? 08/27/2018

ERBB2 levels can be assessed using immunohistochemistry and fluorescence in situ hybridization (FISH) in tissue samples.

How is ERBB2 involved in cell growth and proliferation? 08/07/2018

It forms heterodimers with other ERBB receptors, activating pathways that promote cell growth and survival.

Are there known genetic mutations that lead to ERBB2 overexpression? 02/22/2018

Amplification of the ERBB2 gene leads to its overexpression in cancer cells.

Customer Reviews (3)

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Reviews
05/09/2022

    Worked as expected, satisfied.

    03/16/2022

      Reliable in protein assays.

      09/27/2020

        Quick and reliable shipping.

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