Active Recombinant mouse Abl1, His-tagged

• Cat.No.: Abl1-982M
• Product Overview: Recombinant mouse ABL1 (27-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag.

Specification

• Description: ABL1 protooncogene encodes a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in processes of cell differentiation, cell division, cell adhesion, and stress response. Activity of ABL protein is negatively regulated by its SH3 domain and deletion of the SH3 domain turns ABL1 into an oncogene. Translocation and head-to-tail fusion of the BCR and ABL1 genes is present in many cases of chronic myelogeneous leukemia. The DNA-binding activity of the ubiquitously expressed ABL1 tyrosine kinase is regulated by CDK1-mediated phosphorylation, suggesting a cell cycle function for ABL1.
• Source: Sf9 insect cells using baculovirus
• Species: Mouse
• Tag: His
• Form: Recombinant protein stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
• Bio-activity: 820 nmol/min/mg
• Molecular Mass: ~135 kDa
• Purity: >70%
• Applications: Kinase Assay; Western Blot
• Stability: 1 yr at -70oC from date of shipment.
• Storage: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 ug/ul

Gene Information

• Gene Name: Abl1?c-abl oncogene 1, non-receptor tyrosine kinase [?Mus musculus(house mouse) ]
• Official Symbol: Abl1
• Synonyms: c-abl oncogene 1, non-receptor tyrosine kinase
• Gene ID: 11350
• mRNA Refseq: NM_009594
• Protein Refseq: NP_033724
• UniProt ID: P00520
• Chromosome Location: 2 B; 2 21.86 cM
• Pathway: Alpha6-Beta4 Integrin Signaling Pathway; CDO in myogenesis; Chronic myeloid leukemia
• Function: SH3 domain binding; magnesium ion binding; mitogen-activated protein kinase binding